Thanx Dr.Kris Jett for inviting me as a member. I am presently working on Barcoding of public health imprtant Mosquito species in southern India.I am looking forward to get your valuable suggestion to do better barcoding work. I have already been working on the genotyping of Dengue and Chikungunya viruses prevalent in southern India.
Thank you for such kind invitation, as newbie in dna barcoding works at present I'm dealing with endangered and edible reef species, from fish (napoleon wrasse) and clams (giant clams and abalones) in Seribu Islands, Indonesia.
Dear Kris Jet..
Specially I have not done work on Barcoding. I am specially working on genetics of Bengal tiger like Sequencing and genotyping.
But i am highly interested to do work on Barcoding of wild species from Marine to Mammals.
Initially I have designed a proposal on barcoding of the MAMMELS , Schedule -I species under Wildlife protection act 1972.in the Supervision of my supervisor Dr S.P.Goyal , Scientist F Wildlife Forensic cell wildlife ,Wildlife Institute of India , Dehradun , India I have good expert hand to do lab work.
Please advise me how I can utilize my proposal,
I am looking forward for your kind suggestion and help.
I am Sujeet Singh , working as junior research fellow on tiger genome project India
I have completed my M.Sc. in Biotechnology in 2009. I have designed some proposal work on the bar coding of wild mammals reptiles bird and fish .
I'm hearing lots of good stuff about you. Thanks for the collegial invite. Presently I am away from BIO for two months resting and rehabilitating and back injury. I'll also be taking pictures out in the field whenever I can.
I am involved in the Tree-BOL project. Currently I am undertaking an ambitious but not impossible project of barcoding the trees of Nigeria, numbering some 900 species in around 54 families, spreading over diverse eco-floristic zones: from wetlands to drylands. I may not complain too much on inadequacy of molecular systematics facilities here because I receive ready support from Jodrell Laboratory, Kew (thanks to Prof. Mark Chase) and Masha of Biodiversity Institute of Ontario. I depend on them from extraction to sequencing. Another challenge is not obtaining high quality DNA for sequencing. Masha thinks my samples were not green enough and therefore the low success rate of about 20% from a sample lot of 190 specimens. It was almost frustrating. But after meeting Masha at the Tree-BOL Symposium in New York last April, and talking over the problem of sample preparation and DNA quality, I returned to Nigeria with a stronger determination to prepare better silica-dried samples. But then, the occurrence of several alkaloids in tropical leaves remains an important challenge for DNA barcoding.
One of my students will barcode the invasive trees of Nigeria. We hope to collect cambium in addition to the leaf samples, for comparative purposes.
Meanwhile, I am preparing silica-dried samples to be sent to Masha in tubes. I am concentrating on the two-locus combination, matK and rbcL proposed by the Plant Working Group. But Masha will include other genes with a view to generating data for phylogeny.
I am also interested in barcoding Herbarium samples. Do you know anyone who is keen?