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Universal DNA barcode preferred for animal species identification

A well-informed choice of genetic locus is central to DNA barcoding.Current DNA barcoding in animals involves the use of the 5’ half of themitochondrial cytochrome oxidase 1 gene (CO1) to diagnose and delimit species.However, there is no compelling a priori reason for the exclusive focus on this region,and it has been shown that it performs poorly for certain animal groups. To explore alternative mitochondrial barcoding regions, this research compared the efficacy of the universal CO1 barcode with the other mitochondrial protein-coding genes based on 1,179 mitochondrial genomes of eutherians via bioinformatic analyses. Results from analyses on the number of recovered species, sequence variability within and between species, resolution to taxonomic levels above that of species, and the degree of mutational saturation show that the universal CO1 barcode is a good representative of mitochondrial genes, because the high species-recovery rate (>90%) was similar to that of other mitochondrial genes, and there were no significant differences in intra- or interspecific variability among genes. However, an overlap between intra- and interspecific variability was still problematic for all mitochondrial genes. And any choice of mitochondrial gene for DNA barcoding failed to offer significant resolution at higher taxonomic levels. On the whole, the research suggests that the universal CO1 barcode is preferred among the mitochondrial protein-coding genes as a molecular diagnostic at least for eutherian species identification.

This study was mainly done by researchers from the group led by Dr Chao-Dong Zhu in the Institute of Zoology, Chinese Academy of Sciences. Ms. A-Rong LUO, being the 1st author of the paper, has been one of the assistant professors in the institute. The paper was published in the journal of BMC Genomics. This paper has been the one highly accessed in the journal. The URL is http://www.biomedcentral.com/1471-2164/12/84 .

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Comment by Dr.C.VADIVALAGAN on September 26, 2014 at 5:01am

Greetings!!!

 
Dear sir/Madam,

I am writing to inquire about the availability of postdoctoral positions in your laboratory. Currently, I have completed my doctoral program in the Dept. of Zoology, Bharathiar University, Coimbatore (TN), INDIA. My thesis title mainly focused on the“Molecular identification of butterflies and host –plant association using DNA barcoding with protein - ligand interaction.”


 

In this connection, I have visited you website, I really impressed. Even I also, did some work which mainly focused  on the butterflies and host-plant with special reference to the DNA Barcoding. Hence, I am having considerable amount of research experience in the field of Molecular Biology and Biotechnology aspects with a profound knowledge in Molecular Taxonomy, Genomics & proteomics and Molecular Biology.

 


I am herewith enclosing my curriculum vitae for your kind perusal. If a chance is given, I will discharge my duties to the fullest satisfaction of my superior and other academician


 


Am looking forward to hearing from you.

Thanking you.

with regards.

C. Vadivalagan

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