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Valerie Hofstetter's Page
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Valerie Hofstetter replied to Rebecca Yahr's discussion 'LSU - multiple bands' in the group Fungi"Hi Becky,
Nice to hear from you too...! I tried to modify my answer but apparently it did not work. I wanted to precise that even with dilutions or modifying the PCR program you will not avoid lichen contaminants. This problem is…"
Valerie Hofstetter replied to Rebecca Yahr's discussion 'LSU - multiple bands' in the group Fungi"Me again...
I also tried to avoid contaminant amplification. The major problem is that lichenized fungi have often many more introns than the non-lichenized one and the PCR is biased toward the shorter fragment. With much more template DNA of…"
Valerie Hofstetter replied to Rebecca Yahr's discussion 'LSU - multiple bands' in the group Fungi"Hello Rebecca,
Working on Usnea, I bet you have multiple bands because of lichen endophytes. I always had that problem with ribosomal genes of lichens in the AFTOL project. I am afraid that cloning will be the only solution...
All the…"
Valerie Hofstetter joined Conrad Schoch's group
Fungi
To provide up-to-date information on fungal barcoding and to facilitate communication and the development of collaboration.
Profile Information
- Institution/Organization
- Agroscope Changins-Wädenswil
- Title/Position
- Other
- If Other Title, please specify
- Research associate
- I’m involved in, or am interested in the following barcoding initiatives
- TreeBOL, DNA Barcoding Initiative for Conservation, Data Analysis Working Group
- I’m involved in or interested in the following iBOL Working Groups
- WG1.3 Fungi, WG1.5 Agricultural and Forestry Pests and Their Parasitoids
- Keywords about my barcoding projects (separate each choice with a comma)
- fungi, grapevine, molecular systematics, endophytes
- I’m involved in the following aspects of barcoding:
- Fieldwork/specimen collecting, Labwork/generating barcode sequences, Using barcode data in taxonomy/systematic biology
- Current Project(s)
- Fungal community associated with Vitis vinifera
Phylogeny of Russula and Cantharellus
Fungal diversity in Mayotte.
Comment Wall (1 comment)
- At 12:14pm on November 5, 2010,
Matthew Sileo said… - Hello and welcome!
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