Nice to hear from you too...! I tried to modify my answer but apparently it did not work. I wanted to precise that even with dilutions or modifying the PCR program you will not avoid lichen contaminants. This problem is…"
I also tried to avoid contaminant amplification. The major problem is that lichenized fungi have often many more introns than the non-lichenized one and the PCR is biased toward the shorter fragment. With much more template DNA of…"
Working on Usnea, I bet you have multiple bands because of lichen endophytes. I always had that problem with ribosomal genes of lichens in the AFTOL project. I am afraid that cloning will be the only solution...
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