if it is two clearly visible fragments, than either the primer sequences are not specific enough for the taxon or the annealing temperature is to low. You could try a gradient PCR to check if more stringent temperatures will push the…"
Dear friendsI have chosen a medicinal plant from fabacea family and done barcoding using psbA trnfH primers. but i got 2 different amplicons for that particular primers . Can anyone please suggest me, your valuable comments.See More
I’m involved in, or am interested in the following barcoding initiatives
DNA Barcoding Initiative for Conservation, Plant Working Group, Database Working Group, Data Analysis Working Group
I’m involved in or interested in the following iBOL Working Groups
WG1.5 Agricultural and Forestry Pests and Their Parasitoids, WG4.1 Environmental Barcoding
I’m involved in the following aspects of barcoding:
Fieldwork/specimen collecting, Museum work/specimen curation, Labwork/generating barcode sequences, Management of barcode data, Analysis of barcode data, Using barcode data in taxonomy/systematic biology, Using barcode data for other applications, Organizing/managing barcoding projects, Science policy related to barcoding
Biotechnological studies on some medicinal plants
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