We used the RPB1 primers listed by Conrad (as developed by Ben Hall but modified by Sung?), but also designed a new RPB1C rev primer for Pyronemataceae a bit inside the original primer, which worked well. We sequenced the A-D region…"
For Pezizomycetes I found both DNA quality and specificity of primers matters. DNA extracted from fresh apothecia stored in DNA extraction buffer works best, but well dried, not too old apothecia works as well.
For amplification of…"
Years ago I had this problem with three groups of Pezizaceae. They possessed sequences with short homopolymere regions of 8 to 12 Ts or As in different positions, which caused sequencing problems. I obtained the majority of these sequences…"
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