Keywords about my barcoding projects (separate each choice with a comma)
fish, shellfish, methodology
I’m involved in the following aspects of barcoding:
Fieldwork/specimen collecting, Labwork/generating barcode sequences, Management of barcode data, Analysis of barcode data, Using barcode data in taxonomy/systematic biology, Using barcode data for other applications, Organizing/managing barcoding projects, Science policy related to barcoding
I am happy that you accept my invitation. I am also new here, hope to communicate more frequent after this. I have questions to ask, since you are interested in the methodology - I have problem in using RAG1 as nuclear marker in my PCR work. It's hard to amplify, if did amplify the sequence results was so bad even good bright bands produced during first PCR, and after purification. Why is that so? Do I have to use another primer set for sequencing?