The University of Adelaide will host the first-ever Southern Hemisphere barcode conference, co-organized by CBOL and co-hosted by several biodiversity institutions and initiatives in Australia. The goals of the conference are: highlighting the great progress barcoding has made since the Mexico City Conference in November 2009; learning about barcoding activities in Australia and the region; and bringing barcoders together to exchange ideas and plan new activities. The week’s events will start…See More
If you really do not want to clone, there are several alternative methods to optimize your PCR and reduce your bands.
1) Use less DNA template in your PCR reaction, adding to much DNA could be messing up…"
Could you please tell us what species/genera you are working on?
The optimal method of amplifying loci via PCR can vary per genera, also something like as your PCR mix can matter.
If you can provide us with…"
In the Capnodiales, Primers matter a lot more than DNA quality.
With the standard fRPB2-5F forward primers combined with a Capnodiales designed RPB2 reverse primer and some quality Taq, we have been able to sequence 5 year old samples of…"
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