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Tiago Andrade
  • Male
  • Feira de Santana, Bahia
  • Brazil
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Tiago Andrade's Colleagues

  • Patrícia de Cássia Ruy
  • Alisson Cruz
  • Davi Augusto C. de Almeida
  • Dhivya
  • Huzefa  Raja
  • BD Shenoy
  • Andrew Miller
  • Diego Pignataro
  • Kris Jett

Tiago Andrade's Groups

Tiago Andrade's Discussions

PCR for fungi's COI region (cytochrome oxidase I)
9 Replies

Hi, I'm trying to amplify the region of mitochondrial DNA in conidial fungi that synthesizes protein for cytochrome oxidase I, using primers AHyFu-F and AHyFu-R,  but without success. It's welcome…Continue

Tags: fungi, conidial, AHyFu-F, AHyFu-R, COX1

Started this discussion. Last reply by Tiago Andrade Jan 29, 2011.

 

Tiago Andrade's Page

Latest Activity

Tiago Andrade shared Conrad Schoch's discussion on Facebook
Jul 3, 2012
Tiago Andrade joined Prasanna Kumar's group
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Barcoding Bacteria

To discuss issues pertaining DNA barcoding bacteriaSee More
Apr 23, 2012
Tiago Andrade shared Conrad Schoch's discussion on Facebook
Jan 25, 2012
Tiago Andrade shared Conrad Schoch's discussion on Facebook
Jan 25, 2012
Tiago Andrade shared Conrad Schoch's group on Facebook
Jan 25, 2012
Patrícia de Cássia Ruy and Tiago Andrade are now colleagues
Aug 8, 2011
Tiago Andrade joined Conrad Schoch's group
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Fungi

To provide up-to-date information on fungal barcoding and to facilitate communication and the development of collaboration.
Apr 25, 2011
Tiago Andrade and Dhivya are now colleagues
Apr 20, 2011
Tiago Andrade and Diego Pignataro are now colleagues
Jan 31, 2011
Tiago Andrade replied to Tiago Andrade's discussion 'PCR for fungi's COI region (cytochrome oxidase I)'
"Alright, Isaac.   Thank you for your suggestion.   Thanks for your help too, Diego.   Tiago"
Jan 29, 2011
Isaac Chow replied to Tiago Andrade's discussion 'PCR for fungi's COI region (cytochrome oxidase I)'
"Hi Tiago,   I understand you may try to work out alternative solution for the fungi barcoding, but you may also notice that it is way harder for you to try other regions else apart from ITS, not only because of the major focusing on the ITS,…"
Jan 28, 2011
Tiago Andrade replied to Tiago Andrade's discussion 'PCR for fungi's COI region (cytochrome oxidase I)'
"Look,I know about the sucess of ITS, as I use ITS to amplify my fungi sames too. But, I am using cO1 as a test.  I am testing co1 according Seifert at al (2009). I still do not sequence, but by eletroforesis results, maybe the the amount of…"
Jan 28, 2011
Isaac Chow replied to Tiago Andrade's discussion 'PCR for fungi's COI region (cytochrome oxidase I)'
"Hi Diego,   I understand that it is always easier to amplify with trnH-psbA/ rbcL universal primers and with the matK, it is not always that lucky.   Alignment and re-design conserved primers will be a good move, however, it will only…"
Jan 18, 2011
Diego Pignataro replied to Tiago Andrade's discussion 'PCR for fungi's COI region (cytochrome oxidase I)'
"Hi Isaac, Thanks a lot for ur replay. Yes, I did..how?..well I performed an alignment with a group of peperomias and search the more conservated region (above 500bp) et voilá. BUT I do have problems with my cinchonas samples..not worked it…"
Jan 18, 2011
Isaac Chow replied to Tiago Andrade's discussion 'PCR for fungi's COI region (cytochrome oxidase I)'
"Hi Diego,   If you are concerning the ITS regions, I would suggest the ITS4/5 (White et al, 1990). Further, have you manager to solve your matK amplification that was raised at Mar 2010? If not, I would more than happy to provide some…"
Jan 18, 2011
Diego Pignataro replied to Tiago Andrade's discussion 'PCR for fungi's COI region (cytochrome oxidase I)'
"I´m agree with Isaac. I will try this primers in my fungus sampels next week. Now we are trying to get an appropiate DNA extraction protocol (basidiomicetos) do you suggest one?   I will try with this: Rapid DNA Extraction for Screening…"
Jan 18, 2011

Profile Information

Institution/Organization
State University of Feira de Santana - UEFS
Title/Position
Undergraduate student
Website
http://www.uefs.br/portal
I’m involved in, or am interested in the following barcoding initiatives
DNA Barcoding Initiative for Conservation, Plant Working Group, Database Working Group, Data Analysis Working Group, Leading Labs Network
I’m involved in or interested in the following iBOL Working Groups
WG1.3 Fungi, WG2.1 Barcoding Biotas, WG2.3 Methodological Innovation
Keywords about my barcoding projects (separate each choice with a comma)
fungi, hyphomycetes, phylogenetics, Beltrania, DNA extraction, DNA amplification, PCR, DNA sequencing
I’m involved in the following aspects of barcoding:
Fieldwork/specimen collecting, Museum work/specimen curation, Labwork/generating barcode sequences, Management of barcode data, Analysis of barcode data, Using barcode data in taxonomy/systematic biology, Using barcode data for other applications, Organizing/managing barcoding projects, Science policy related to barcoding
Current Project(s)
Barcode dos microfungos do Semi-árido da Bahia depositados na CCMB
(Barcode of the microfungi from Semi-arid of the Bahia deposited in thw CCMB)

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At 9:51am on March 8, 2010, Matthew Sileo said…
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