That the common problem with ABI sequencers. Check ur chromatograms and make sure its free from mis-reading before sequence extraction.
If the sequence of species is available in NCBI, make use of it for alignment.
if still the…"
I’m involved in, or am interested in the following barcoding initiatives
Mosquito Barcoding Initiative, Tephritid Barcoding Initiative, TreeBOL, DNA Barcoding Initiative for Conservation, Database Working Group, Data Analysis Working Group, Leading Labs Network
I’m involved in or interested in the following iBOL Working Groups
WG1.5 Agricultural and Forestry Pests and Their Parasitoids, WG1.6 Pollinators, WG2.2 Museum Life, WG2.3 Methodological Innovation
Keywords about my barcoding projects (separate each choice with a comma)
Agriculture important lepidopterans
I’m involved in the following aspects of barcoding:
Fieldwork/specimen collecting, Museum work/specimen curation, Labwork/generating barcode sequences, Management of barcode data, Analysis of barcode data, Using barcode data in taxonomy/systematic biology, Using barcode data for other applications
I am working on Agriculture important lepidopterans
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