Praveen's Page

A group for anyone interested or involved in barcoding beetles. The group is intended to facilitate collaboration, share experiences and ideas among researchers.

Fish Barcode of Life Campaign

Come share protocols and troubleshooting experiences, compare techniques, and discuss collaborative efforts.

Dear all, I am hereby going to post the experiments performed by me on different kind of insects.To start with following are the names of the insects collected and subjected for the extraction. 1. Red ant, 2. Chilo sp., 3. Beetle A. Procedure for extraction of DNA Cut remove the head of the insects in different PCR tubes. Crush it well with the pestle.Add 10ul of Express extract buffer.Add 2U of Kapa Express extract enzyme 2ulAdd nuclease free water to make up the reaction volume to…See More

Dear all, Its too important for anyone who is going for sequencing because the purification of post PCR product is important so as to get the proper sequencing done otherwise its a bad result indeed.Basicall all the labs would use conventional methods of using the reagents and its a time consumption and also consistency would not be there. Recently i have been doing some purification using a "Tip". Yes its all in the Tip i.e., micropipette tip. It would hardly take about a minute to use the tip…See More