"Hey Shashank
That the common problem with ABI sequencers. Check ur chromatograms and make sure its free from mis-reading before sequence extraction.
If the sequence of species is available in NCBI, make use of it for alignment.
if still the…"
international online community for dna barcoding professionals
-
Prasanna Kumar
- Male
- Tamil Nadu
- India
- Blog Posts
- Discussions (8)
- Events
- Groups (8)
- Photos (21)
- Photo Albums
- Videos
Prasanna Kumar's Groups
-
Polar Life
23 members
-
BOLD Systems
86 members
-
Barcoding Bacteria
9 members
-
Problems in DNA barcoding
58 members
-
Fungi
103 members
Prasanna Kumar's Discussions
DNA barcoding Bacteria
3 Replies
Hi hope all had blast in fourth conference. As dna barcoding technology have been expanded and it almost touched every aspects of tree of life, one group that remained isolated is bacteria. Although…Continue
Tags: barcodes, dna, bacteria, barcoding, prokaryotes
Started this discussion. Last reply by Prasanna Kumar Dec 10, 2011.
Prasanna Kumar's Page
Latest Activity
Prasanna Kumar joined Megan A. Milton's group
BOLD Systems
The Barcode of Life Data Systems (BOLD) is an online workbench that aids collection, management, analysis, and use of DNA barcodes. See More
Prasanna Kumar replied to Prasanna Kumar's discussion 'DNA barcoding Bacteria'"@ Dr. Hosam Osama Elansary
Nice to know we are in same track and facing same problem. Please do check out "marine barcoding" group for my post…"
Hosam Osama Elansary replied to Prasanna Kumar's discussion 'DNA barcoding Bacteria'"We are starting a barcoding project for Bacteria in Egypt and we dont know from where to start, which genes?"
Vijaykumar Yogesh Muley replied to Prasanna Kumar's discussion 'DNA barcoding Marine Bacteria' in the group Marine Barcoding"Dear Prasanna Kumar,
I was talking about generalized approach and certainly my comments would be wrong in case of within species variations. As shown in figure attached herewith, Variations among species are evident using rpoB as compared to 16S…"
Prasanna Kumar replied to Prasanna Kumar's discussion 'DNA barcoding Marine Bacteria' in the group Marine Barcoding"@ Dear Vijaykumar Yogesh
Its nice to see your reply. For past few years many papers have produced enough evidence to reject 16s rRNA as taxonomic marker, as it has less variations within species. Variations within multiple 16s gene copies occurring…"
Profile Information
- Institution/Organization
- Faculty of Marine Science, Annamalai University
- Title/Position
- Doctoral student
- I’m involved in, or am interested in the following barcoding initiatives
- FISH-BOL, Marine Barcode of Life (MarBOL), DNA Barcoding Initiative for Conservation
- I’m involved in or interested in the following iBOL Working Groups
- WG1.1 Vertebrates, WG1.3 Fungi, WG1.8 Marine Bio-Surveillance, WG2.1 Barcoding Biotas, WG4.1 Environmental Barcoding
- Keywords about my barcoding projects (separate each choice with a comma)
- marine fin fishes, bacteria, eggs and larvae
- I’m involved in the following aspects of barcoding:
- Fieldwork/specimen collecting, Labwork/generating barcode sequences, Management of barcode data, Analysis of barcode data, Using barcode data in taxonomy/systematic biology, Using barcode data for other applications
- Current Project(s)
- Detecting the mobility of eggs and larvae through DNA barcodes in marine environment & defining barcodes for marine bacteria
Prasanna Kumar's Photos
© 2012 Created by Matthew Fisher.
Powered by
