"Dear Swapnil,
Thank you for that advice.
Sincerely,
Matt"
international online community for dna barcoding professionals
Matthew Bowser's Groups
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Barcoding All Lepidoptera
44 members
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Mosquito Barcoding Initi…
29 members
Matthew Bowser's Page
Latest Activity
Matthew Bowser replied to Matthew Bowser's discussion 'DNA-friendly killing agents' in the group Barcoding All Lepidoptera
Gaikwad Swapnil Sopan replied to Matthew Bowser's discussion 'DNA-friendly killing agents' in the group Barcoding All Lepidoptera"Dear Matthew,
According to my personal experience, no much damage to DNA is done if you keep specimen for one day in your collection kit. Yes of course after returning to the lab one should keep them at zero degrees or minus twenty.…"
Matthew Bowser replied to Matthew Bowser's discussion 'DNA-friendly killing agents' in the group Barcoding All Lepidoptera"Thank you for those recommendations. I'll be dealing more with small moths than larger Lepidoptera. Freezing will not be available to me in the field, although I suppose I could bring dry ice. What can be used in traps, e.g.…"
Mansi Mungee replied to Matthew Bowser's discussion 'DNA-friendly killing agents' in the group Barcoding All Lepidoptera"I agree with Swapnil. Gentle squeeze on the thorax works well and does not cause any intermediate or downstream DNA damage. We have used killing jars (with chloroform soaked cotton) also for collecting and killing Moths and the DNA quality or yield…"
Gaikwad Swapnil Sopan replied to Matthew Bowser's discussion 'DNA-friendly killing agents' in the group Barcoding All Lepidoptera"Yes freezing is better potion. But I think its better to kill these beauties by pressing their abdomen or pressing between head and thorax once you catch them. Hoever later one is better otion. I don't recommend chemical killing method as it…"
Matthew Bowser added a discussion to the group Barcoding All Lepidoptera
DNA-friendly killing agents
What are the best DNA-friendly killing agents for Lepidoptera? Freezing and cyanide are the available options I have read about, but what about ammonium carbonate?
Matthew Bowser joined Rodolphe Rougerie's groupBarcoding All Lepidoptera
Learn more and find out how to join efforts toward assembling a comprehensive DNA barcode library for all species of butterflies and moths
Matthew Bowser commented on Yvonne Linton's group 'Mosquito Barcoding Initiative'"Monitoring of Culicidae and other terrestrial arthropod taxa using next-gen (environmental sampling) DNA barcoding methods is the long-term objective of a mini-bioblitz we are planning for this summer. This field season, we will be sampling…"
Matthew Bowser commented on Yvonne Linton's group 'Mosquito Barcoding Initiative'"I am looking for help with inventorying and obtaining DNAbarcodes of mosquitoes from the Kenai National Wildlife Refuge thissummer. This is part of a larger arthropod inventory initiative on therefuge, but the Culicidae is a priority group…"
Matthew Bowser replied to Kris Jett's discussion 'Introductions'"Hello,
My name is Matt Bowser, Entomologist at the Kenai National Wildlife Refuge, Soldotna, Alaska. I am not a DNA barcoding expert, but I am hoping to make use of developing DNA barcoding methods for use in inventorying and monitoring…"
Profile Information
- Institution/Organization
- Kenai National Wildlife Refuge
- Title/Position
- Government official
- I’m involved in, or am interested in the following barcoding initiatives
- Mosquito Barcoding Initiative, Tephritid Barcoding Initiative
- I’m involved in or interested in the following iBOL Working Groups
- WG1.6 Pollinators, WG1.7 Freshwater Bio-Surveillance, WG1.9 Terrestrial Bio-Surveillance, WG2.1 Barcoding Biotas
- I’m involved in the following aspects of barcoding:
- Fieldwork/specimen collecting, Museum work/specimen curation
- Current Project(s)
- Building a DNA barcode library for terrestrial and freshwater aquatic arthropods of the Kenai National Wildlife Refuge, Alaska.
Comment Wall (1 comment)
- At 12:25pm on December 8, 2010,
Matthew Sileo said… - Hello and welcome!
Thank you for taking the time to join. Here are a few things that you can do in the community.
After completing your online profile, please add yourself to the member map. Next, invite your colleagues and search our members for people you know and invite them to be your colleague in the network. Or browse our Forum and ask a question.
Please join a Group or start your own, as groups are a great way to express your special interest, be it a taxonomic group, a regional or organizational affiliation.
Have a question? Ask one of our hosts.
A few more...
Please contribute to our Blog. Simply choose “Add a Blog Post” under Blogs.
If you already have a blog, you can share it through RSS either on your page or on our rss pages. Just ask me how.
Or create a group and invite colleagues to join. Use “Send Message to Group” to email everyone in your group, start discussions specific to your group’s interests, add widgets and more.
For assistance, don’t hesitate to contact me.
Again, thank you for joining our growing online community. I look forward to connecting with you soon.
-Matt Sileo
© 2012 Created by Matthew Fisher.
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