international online community for dna barcoding professionals

Marieka Gryzenhout
  • Female
  • Bloemfontein
  • South Africa
  • Blog Posts
  • Discussions
  • Events
  • Groups (10)
  • Photos (1)
  • Photo Albums
  • Videos

Marieka Gryzenhout's Colleagues

  • Mariette Truter
  • Michelle van der Bank

Marieka Gryzenhout's Page

Latest Activity

Marieka Gryzenhout added a discussion to the group Environmental Barcoding

Saturation of a run

HelloNIce to see some of you at Adelaide, and the research is awesome.  We pyrosequence fungal endophytes (fungi occurring inside plant tissues) directly from the plant material, and analyse the resulting mini-barcodes.   Is there a method to see that in your run, you have completely saturated a lane, so there may be missing data in the output, or that all of your products are there? This is difficult because we are doing this for the first time, so there is no benchmark to compare…See More
Feb 10, 2012
Bevan Weir replied to Marieka Gryzenhout's discussion 'Collecting macrofungi for barcoding' in the group Fungi
"Hi,   What I have done in the past is put a small amount of fresh tissue in a buffer (e.g. CTAB) in a 1.5 mL tube, then dry down the rest of the specimen for the herbarium. You will need to have collection codes to link the samples of course.…"
Oct 3, 2011
Marieka Gryzenhout added a discussion to the group Fungi

Collecting macrofungi for barcoding

Hi all I am about to go on an official barcoding trip in South Africa. Besides the small ascomycetes that is more my specialty, I will also collect some larger fungi.  THis trip has a public profile, so the larger fungi will be there for the people to better understand and to attract their attention.  Because I want to do it properly, are there any do's and dont's when I collect the larger fungi and preparing them for barcoding, e.g. how much tissue must I put in directly, I should probably dry…See More
Oct 3, 2011
Michelle van der Bank and Marieka Gryzenhout are now colleagues
Nov 15, 2010
Marieka Gryzenhout posted a photo
Nov 15, 2010
Marieka Gryzenhout and Mariette Truter are now colleagues
Nov 15, 2010
Marieka Gryzenhout joined Kris Jett's group

Field Operations

Creating barcode reference records often begins with a collecting event in the field, but without the right techniques the specimen may never qualify as a high-quality barcode record. Come exchange information on field protocols!See More
Nov 15, 2010
Marieka Gryzenhout joined Brian L Fisher's group


Join this group to discuss efforts to barcode life in Madagascar.
Nov 15, 2010
Marieka Gryzenhout joined Kris Jett's group

Lab Operations

Come share protocols and troubleshooting experiences, compare techniques, and discuss collaborative efforts.
Nov 15, 2010
Marieka Gryzenhout joined Gaikwad Swapnil Sopan's group

Problems in DNA barcoding

Dear all,This group is intend to solve problems occurred while analyzing barcode sequences and using appropriate method of analysis.See More
Nov 15, 2010
Marieka Gryzenhout joined Conrad Schoch's group


To provide up-to-date information on fungal barcoding and to facilitate communication and the development of collaboration.
Nov 15, 2010
Marieka Gryzenhout joined Lorenzo Lombard's group


For all research groups active in barcode development and/or research in Europe and developing countries in Africa.
Nov 15, 2010
Marieka Gryzenhout joined Mehrdad Hajibabaei's group
Nov 15, 2010
Marieka Gryzenhout is now a member of
Nov 15, 2010

Profile Information

University of the Free State
Researcher/Faculty member
I’m involved in, or am interested in the following barcoding initiatives
DNA Barcoding Initiative for Conservation, Database Working Group, Data Analysis Working Group
I’m involved in or interested in the following iBOL Working Groups
WG1.3 Fungi, WG2.1 Barcoding Biotas
Keywords about my barcoding projects (separate each choice with a comma)
endophytes, fungal pathogens
I’m involved in the following aspects of barcoding:
Fieldwork/specimen collecting, Labwork/generating barcode sequences, Management of barcode data, Analysis of barcode data, Using barcode data in taxonomy/systematic biology, Using barcode data for other applications
Current Project(s)
Barcoding and pyrosequencing of fungal tree endophytes
Barcoding of plant pathogens

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Comment Wall (1 comment)

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At 3:11am on November 23, 2015, Barron Moon said…

es bueno verte aquí. ¿Si podría tener un deseo, quisiera despertar todos los días con el sonido de su respiración en mi cuello, eres hermosa!, podemos ser amigos? ¿Mi nombre es luna Barron, soy un Soldier.Please,Can de UN marino tengo su ID de correo electrónico para que podamos comunicarnos desde allí? Mi id de correo electrónico es (barronmoon(AT) en espero con interés escuchar de usted.
Bye con amor
Barron Luna.

is nice meeting you here.If I could have just one wish,I would wish to wake up everyday to the sound of your breath on my neck,you are beautiful!can we be friends?My name is Barron Moon,i am a UN marine Soldier.Please,Can i have your email ID so that we can communicate from there? My email id is (barronmoon(AT) I look forward hearing from you.
Bye With love
Barron Moon.



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