international online community for dna barcoding professionals
Le Ngoc Trieu's Page
Latest Activity
Le Ngoc Trieu added a discussion to the group Plants
PCR condition for amplication matK gene with pair of primers trnK3914F and trnK2R in Panax genus
I'm investigating phylogenetic in Panax genus (Araliaceae). I tried some published PCR condition for amplification matK gene with pair of specific primers trnK3914F (5’-TGG GTT GCT AAC TCA ATG G-3’) and trnK2R (5'-AAC TAG TCG GAT GGA GTA G-3') (in attached files) but I could not recognize the desired band. My problem is no observed band. I had tried gradient PCR with Tm ranged from 45 to 68 deegrees also, The size of desired band is 1509 - 1512bp, so I also try to increase the time for strain…See More
Profile Information
- Institution/Organization
- Centre for Application nuclear technique
- Title/Position
- Researcher/Faculty member
- I’m involved in, or am interested in the following barcoding initiatives
- TreeBOL, DNA Barcoding Initiative for Conservation, Plant Working Group, Database Working Group, Data Analysis Working Group
- I’m involved in or interested in the following iBOL Working Groups
- WG1.1 Vertebrates, WG1.2 Land Plants, WG1.3 Fungi, WG1.5 Agricultural and Forestry Pests and Their Parasitoids
- I’m involved in the following aspects of barcoding:
- Fieldwork/specimen collecting, Analysis of barcode data, Using barcode data in taxonomy/systematic biology
- Current Project(s)
- Taxonomy in genus Panax
© 2013 Created by Matthew Fisher.
Powered by
My Comment Wall
You need to be a member of Connect.BarcodeofLife.net to add comments!
Join Connect.BarcodeofLife.net