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Le Ngoc Trieu
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  • Vietnam
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  • k.sathis kumar

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Maonian Xu replied to Le Ngoc Trieu's discussion 'Can anyone suggest me about PCR condition for amplication matK gene with pair of primers trnK3914F and trnK2R in Panax genus' in the group Medicinal plants
"Hi,  I did not use the trnK primer pairs, but matK 390F and matK 1326R. You can also use other pairs like 472F and 1248R or xF and MALPRI. The protocol is the same as the protocol attached using Phusion polymerase. Good luck! Maonian"
Mar 25
Le Ngoc Trieu and k.sathis kumar are now colleagues
Mar 25, 2012
Le Ngoc Trieu added 2 discussions to the group Medicinal plants
Mar 6, 2012
Le Ngoc Trieu added a discussion to the group Plants

PCR condition for amplication matK gene with pair of primers trnK3914F and trnK2R in Panax genus

I'm investigating phylogenetic in Panax genus (Araliaceae). I tried some published PCR condition for amplification matK gene with pair of specific primers trnK3914F (5’-TGG GTT GCT AAC TCA ATG G-3’) and trnK2R (5'-AAC TAG TCG GAT GGA GTA G-3') (in attached files) but I could not recognize the desired band. My problem is no observed band. I had tried gradient PCR with Tm ranged from 45 to 68 deegrees also, The size of desired band is 1509 - 1512bp, so I also try to increase the time for strain…See More
Mar 6, 2012
Le Ngoc Trieu joined Karen James's group
Mar 6, 2012
Le Ngoc Trieu joined Kalpesh Ishnava's group

Medicinal plants

Plants-Medicinal Plants
Mar 5, 2012
Le Ngoc Trieu is now a member of
Mar 5, 2012

Profile Information

Centre for Application nuclear technique
Researcher/Faculty member
I’m involved in, or am interested in the following barcoding initiatives
TreeBOL, DNA Barcoding Initiative for Conservation, Plant Working Group, Database Working Group, Data Analysis Working Group
I’m involved in or interested in the following iBOL Working Groups
WG1.1 Vertebrates, WG1.2 Land Plants, WG1.3 Fungi, WG1.5 Agricultural and Forestry Pests and Their Parasitoids
I’m involved in the following aspects of barcoding:
Fieldwork/specimen collecting, Analysis of barcode data, Using barcode data in taxonomy/systematic biology
Current Project(s)
Taxonomy in genus Panax

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