"Hi Rebecca,
We have this problem from time to time and it's been due to intron presence of variable sizes. Usually, the introns are adjacent to the LR5 primer. To avoid excising PCR bands or cloning, you could try sequencing from the left…"
international online community for dna barcoding professionals
Karen W. Hughes's Page
Latest Activity
Karen W. Hughes joined Conrad Schoch's group
Fungi
To provide up-to-date information on fungal barcoding and to facilitate communication and the development of collaboration.
Profile Information
- Institution/Organization
- University of Tennessee, Knoxville
- Title/Position
- Researcher/Faculty member
- Website
- http://www.bio.utk.edu/mycology/
- I’m involved in or interested in the following iBOL Working Groups
- WG1.3 Fungi
- I’m involved in the following aspects of barcoding:
- Fieldwork/specimen collecting, Museum work/specimen curation, Labwork/generating barcode sequences, Analysis of barcode data, Using barcode data in taxonomy/systematic biology
- Current Project(s)
- ATBI Great Smoky Mountains National Park, biogeography of agaric fungi, phylogenetic relationships
Comment Wall (1 comment)
- At 2:12pm on October 6, 2010,
Matthew Sileo said… - Hello and welcome!
Thank you for taking the time to join. Here are a few things that you can do in the community.
After completing your online profile, please add yourself to the member map. Next, invite your colleagues and search our members for people you know and invite them to be your colleague in the network. Or browse our Forum and ask a question.
Please join a Group or start your own, as groups are a great way to express your special interest, be it a taxonomic group, a regional or organizational affiliation.
Have a question? Ask one of our hosts.
A few more...
Please contribute to our Blog. Simply choose “Add a Blog Post” under Blogs.
If you already have a blog, you can share it through RSS either on your page or on our rss pages. Just ask me how.
Or create a group and invite colleagues to join. Use “Send Message to Group” to email everyone in your group, start discussions specific to your group’s interests, add widgets and more.
For assistance, don’t hesitate to contact me.
Again, thank you for joining our growing online community. I look forward to connecting with you soon.
-Matt Sileo
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