"Good luck, I hope the advice helped!"
international online community for dna barcoding professionals
Handan's Groups
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Lab Operations
65 members
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CBOL Leading Labs Networ…
46 members
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Problems in DNA barcoding
69 members
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Plants
113 members
Handan's Page
Latest Activity
Paula replied to Handan's discussion 'MatK gene region in angiosperm' in the group Problems in DNA barcoding
Handan replied to Handan's discussion 'MatK gene region in angiosperm' in the group Problems in DNA barcoding"I understood, I will try to change my temperature. I hope, I get a good amplification...
thank you so much .."
Handan replied to Handan's discussion 'MatK gene region in angiosperm' in the group Problems in DNA barcoding"Hi,
I use primers trnK-2R and trnK3914F ... ;Is it same your primers??
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Paula replied to Handan's discussion 'MatK gene region in angiosperm' in the group Problems in DNA barcoding"I read in an article that DMSO lowers the annealing temperature of your primers, so if you add DMSO, you will have to adjust your cycling conditions.
I use primers 1248R (Tm 52.5C) and 472F (Tm 57.8C) and my PCR annealing temp is 53.7 when I…"
Rasika Bhagwat replied to Handan's discussion 'MatK gene region in angiosperm' in the group Problems in DNA barcoding"Hi
Which primers you are using, becoz I am also facing the same problem.
Rasika
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Handan added a discussion to the group Problems in DNA barcoding
MatK gene region in angiosperm
Dear all,I am currently working on DNA Barcoding of some plants (belonging to Angiosperm). Fistly ı did not use DMSO for MatK gene region. But sequence results are not good and then ı use the DMSO, but so now non-spesific band is occurring. Does anyone know why this might be? How can I do ??See More
Handan joined Kris Jett's group
Lab Operations
Come share protocols and troubleshooting experiences, compare techniques, and discuss collaborative efforts.
Handan joined Kris Jett's group
CBOL Leading Labs Network (LLN)
The CBOL Leading Labs Network (LLN) was created in 2007 as a network of barcoding labs around the world dedicated to sharing technical information on barcoding and providing training and resources for new barcoding researchers and labs.
Handan joined Gaikwad Swapnil Sopan's groupProblems in DNA barcoding
Dear all,This group is intend to solve problems occurred while analyzing barcode sequences and using appropriate method of analysis.See More
Profile Information
- Institution/Organization
- Erciyes Univetsity
- Title/Position
- Doctoral student
- I’m involved in, or am interested in the following barcoding initiatives
- FISH-BOL, TreeBOL, DNA Barcoding Initiative for Conservation, Plant Working Group, Database Working Group, Data Analysis Working Group, Leading Labs Network
- I’m involved in or interested in the following iBOL Working Groups
- WG1.2 Land Plants, WG1.3 Fungi, WG1.5 Agricultural and Forestry Pests and Their Parasitoids, WG1.6 Pollinators, WG1.7 Freshwater Bio-Surveillance, WG2.1 Barcoding Biotas, WG2.4 Paleobarcoding, WG3.2 Mirrors, WG4.1 Environmental Barcoding, WG4.2 Mobile Barcoding
- I’m involved in the following aspects of barcoding:
- Fieldwork/specimen collecting, Museum work/specimen curation, Labwork/generating barcode sequences, Management of barcode data, Analysis of barcode data, Using barcode data in taxonomy/systematic biology, Using barcode data for other applications, Organizing/managing barcoding projects, Science policy related to barcoding, Other
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