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Gillian Dean's Colleagues

  • Dr A P Singh
  • Dhivya
  • Cam Webb
  • Kris Jett

Gillian Dean's Groups

 

Gillian Dean's Page

Latest Activity

Gillian Dean replied to Sribash Roy's discussion 'DNA extraction from herbarium samples' in the group Plants
"Hi Sribash: this is going to be very difficult he DNA is likely to be very degraded as the specimens are old and they were treated with Hg. I think that Qiagen make some kits than are supposed to help with difficult samples like these (they were…"
Jul 20, 2011
Gillian Dean is now colleagues with Dhivya and Dr A P Singh
Jul 18, 2011
Gillian Dean replied to Gillian Dean's discussion 'Outsourcing DNA extraction and sequencing' in the group Lab Operations
"Hi Isaac - sorry, for some reason I didn't get a notification that you replied to this thread. Thanks too Dr Singh for your suggestion. We are working with the Canadian Centre for DNA Barcoding at the moment, but we may need more sequencing…"
Jul 17, 2011
Dr A P Singh replied to Gillian Dean's discussion 'Outsourcing DNA extraction and sequencing' in the group Lab Operations
"You can contact Dr George Thomas, Scientist, Rajiv Gandhi Center of Biotechnology, Trivandrum, Kerala for accomplishing the task on payment basis. You can go to the website, download per forma for sending samples and payment Dr A P Singh"
Jul 16, 2011
Gillian Dean replied to Karen James's discussion 'Plant barcoding primers and protocols' in the group Plants
"There is no particular method for silica dried specimens - best to use whatever method you use for fresh material. There could also be problems if your silica material was not dried or stored properly, quick drying is essential (lots of silica and a…"
Jun 28, 2011
Gillian Dean replied to Karen James's discussion 'Plant barcoding primers and protocols' in the group Plants
"No, it didn't get through - but now i have seen it :) interesting to know what groups you work on, thanks!"
Jun 28, 2011
Gillian Dean replied to Karen James's discussion 'Plant barcoding primers and protocols' in the group Plants
"Great, I will check it out. I do like BioLine products. What plants families are you working on? We have a barcoding project which has a real range of families so we need something that works across the board. Thanks!"
Jun 14, 2011
Gillian Dean replied to Karen James's discussion 'Plant barcoding primers and protocols' in the group Plants
"Hi Chrissen - thanks for the reply. I took a look at the sequencing facility, thanks. I'd be glad to hear what kit you are using - I had a small samples of that kit but it was hard to determine how it compared to Qiagen with just a few…"
Jun 14, 2011
Isaac Chow replied to Gillian Dean's discussion 'Outsourcing DNA extraction and sequencing' in the group Lab Operations
"Hi Gill,   Here replying from a commercial lab in Hong Kong, we use in house method for DNA extraction and usually success except few known species where usually with PCR inhibitors.   I would suggest you to try resin based method together…"
Jan 18, 2011
Patricia Escalante replied to Gillian Dean's discussion 'electrophoresis equipment' in the group Lab Operations
"thank you, I got it. Patricia Escalante"
Dec 19, 2010
Gillian Dean added a discussion to the group Lab Operations
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electrophoresis equipment

 i got an email from someone asking about the type of wire to use in a gel tank - it is platinum wire that should be used. for some reason i cant find the post that was in the email in lab operations, maybe someone can help with this problem? best, GillSee More
Dec 18, 2010
Gillian Dean replied to Karen James's discussion 'Plant barcoding primers and protocols' in the group Plants
"We are looking into possibilities for generating barcode data from 3500 Indonesian plant species and we are considering sending our samples out for DNA extracting and sequencing. Does anyone have suggestions for service labs who could help…"
Oct 5, 2010
Gillian Dean replied to David E. Schindel's discussion 'Sharing Lab Protocols' in the group Lab Operations
"I think this is a great idea - it would help answer my new discussion thread. We're in the process of preparing our experiences of doing barcoding in a small lab in Indonesia and I'd be happy to share information with people."
Oct 5, 2010
Gillian Dean added a discussion to the group Lab Operations
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Outsourcing DNA extraction and sequencing

We are looking into possibilities for generating barcode data from 3500 Indonesian plant species and we are considering sending our samples out for DNA extracting and sequencing.Does anyone have suggestions for service labs who could help us?Also, if we were to do the work in Indonesia, I want to move to a kit that uses column-based extraction rather than phenol-chloroform protocol. DO people have suggestions for kits that are good for a wide range of species? I usually use Qiagen, but they are…See More
Oct 5, 2010
Gillian Dean just added their location.
Sep 24, 2010
Gillian Dean joined Kris Jett's group
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Lab Operations

Come share protocols and troubleshooting experiences, compare techniques, and discuss collaborative efforts.
Sep 20, 2010

Profile Information

Institution/Organization
University of BC
Title/Position
Other
If Other Title, please specify
Honorary RA
Website
http://www.gilldean.com
I’m involved in, or am interested in the following barcoding initiatives
TreeBOL, DNA Barcoding Initiative for Conservation, Plant Working Group
I’m involved in or interested in the following iBOL Working Groups
WG1.2 Land Plants
I’m involved in the following aspects of barcoding:
Labwork/generating barcode sequences, Management of barcode data, Analysis of barcode data, Using barcode data in taxonomy/systematic biology, Using barcode data for other applications, Organizing/managing barcoding projects
Current Project(s)
Xmalesia http://phylodiversity.net/xmalesia/

Comment Wall (1 comment)

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At 10:03am on September 21, 2010, Matthew Sileo said…
Hello and welcome!

Thank you for taking the time to join. Here are a few things that you can do in the community.

After completing your online profile, please add yourself to the member map. Next, invite your colleagues and search our members for people you know and invite them to be your colleague in the network. Or browse our Forum and ask a question.

Please join a Group or start your own, as groups are a great way to express your special interest, be it a taxonomic group, a regional or organizational affiliation.

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A few more...
Please contribute to our Blog. Simply choose “Add a Blog Post” under Blogs.
If you already have a blog, you can share it through RSS either on your page or on our rss pages. Just ask me how.

Or create a group and invite colleagues to join. Use “Send Message to Group” to email everyone in your group, start discussions specific to your group’s interests, add widgets and more.

For assistance, don’t hesitate to contact me.

Again, thank you for joining our growing online community. I look forward to connecting with you soon.

-Matt Sileo
 
 
 

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