"Hi Sribash: this is going to be very difficult he DNA is likely to be very degraded as the specimens are old and they were treated with Hg. I think that Qiagen make some kits than are supposed to help with difficult samples like these (they were…"
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Gillian Dean's Page
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Gillian Dean replied to Sribash Roy's discussion 'DNA extraction from herbarium samples' in the group Plants
Gillian Dean replied to Gillian Dean's discussion 'Outsourcing DNA extraction and sequencing' in the group Lab Operations"Hi Isaac - sorry, for some reason I didn't get a notification that you replied to this thread. Thanks too Dr Singh for your suggestion. We are working with the Canadian Centre for DNA Barcoding at the moment, but we may need more sequencing…"
Dr A P Singh replied to Gillian Dean's discussion 'Outsourcing DNA extraction and sequencing' in the group Lab Operations"You can contact Dr George Thomas, Scientist, Rajiv Gandhi Center of Biotechnology, Trivandrum, Kerala for accomplishing the task on payment basis. You can go to the website, download per forma for sending samples and payment
Dr A P Singh"
Gillian Dean replied to Karen James's discussion 'Plant barcoding primers and protocols' in the group Plants"There is no particular method for silica dried specimens - best to use whatever method you use for fresh material. There could also be problems if your silica material was not dried or stored properly, quick drying is essential (lots of silica and a…"
Gillian Dean replied to Karen James's discussion 'Plant barcoding primers and protocols' in the group Plants"No, it didn't get through - but now i have seen it :) interesting to know what groups you work on, thanks!"
Gillian Dean replied to Karen James's discussion 'Plant barcoding primers and protocols' in the group Plants"Great, I will check it out. I do like BioLine products.
What plants families are you working on? We have a barcoding project which has a real range of families so we need something that works across the board.
Thanks!"
Gillian Dean replied to Karen James's discussion 'Plant barcoding primers and protocols' in the group Plants"Hi Chrissen - thanks for the reply.
I took a look at the sequencing facility, thanks.
I'd be glad to hear what kit you are using - I had a small samples of that kit but it was hard to determine how it compared to Qiagen with just a few…"
Isaac Chow replied to Gillian Dean's discussion 'Outsourcing DNA extraction and sequencing' in the group Lab Operations"Hi Gill,
Here replying from a commercial lab in Hong Kong, we use in house method for DNA extraction and usually success except few known species where usually with PCR inhibitors.
I would suggest you to try resin based method together…"
Patricia Escalante replied to Gillian Dean's discussion 'electrophoresis equipment' in the group Lab Operations"thank you, I got it. Patricia Escalante"
Gillian Dean added a discussion to the group Lab Operations
electrophoresis equipment
i got an email from someone asking about the type of wire to use in a gel tank - it is platinum wire that should be used. for some reason i cant find the post that was in the email in lab operations, maybe someone can help with this problem? best, GillSee More
Gillian Dean replied to Karen James's discussion 'Plant barcoding primers and protocols' in the group Plants"We are looking into possibilities for generating barcode data from 3500 Indonesian plant species and we are considering sending our samples out for DNA extracting and sequencing.
Does anyone have suggestions for service labs who could help…"
Gillian Dean replied to David E. Schindel's discussion 'Sharing Lab Protocols' in the group Lab Operations"I think this is a great idea - it would help answer my new discussion thread. We're in the process of preparing our experiences of doing barcoding in a small lab in Indonesia and I'd be happy to share information with people."
Gillian Dean added a discussion to the group Lab OperationsOutsourcing DNA extraction and sequencing
We are looking into possibilities for generating barcode data from 3500 Indonesian plant species and we are considering sending our samples out for DNA extracting and sequencing.Does anyone have suggestions for service labs who could help us?Also, if we were to do the work in Indonesia, I want to move to a kit that uses column-based extraction rather than phenol-chloroform protocol. DO people have suggestions for kits that are good for a wide range of species? I usually use Qiagen, but they are…See More
Gillian Dean joined Kris Jett's groupLab Operations
Come share protocols and troubleshooting experiences, compare techniques, and discuss collaborative efforts.
Profile Information
- Institution/Organization
- University of BC
- Title/Position
- Other
- If Other Title, please specify
- Honorary RA
- Website
- http://www.gilldean.com
- I’m involved in, or am interested in the following barcoding initiatives
- TreeBOL, DNA Barcoding Initiative for Conservation, Plant Working Group
- I’m involved in or interested in the following iBOL Working Groups
- WG1.2 Land Plants
- I’m involved in the following aspects of barcoding:
- Labwork/generating barcode sequences, Management of barcode data, Analysis of barcode data, Using barcode data in taxonomy/systematic biology, Using barcode data for other applications, Organizing/managing barcoding projects
- Current Project(s)
- Xmalesia http://phylodiversity.net/xmalesia/
Comment Wall (1 comment)
- At 10:03am on September 21, 2010,
Matthew Sileo said… - Hello and welcome!
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