"You should chat with the folks at Guelph (especially Natalia Ivanova). We use salts as indicated previously to deal with the polysaccharides, but she got us using acetone to clean up the polyphenolics, which saved us a lot of time and money on…"
"What lineage are you working on? In the algal world we have significant challenges owing to polysaccharides co-extracting with the DNA. In our lab we have found that the best solution is to add potassium acetate to red algal extraction buffers…"
"A recent issue of Cryptogamie Algologie has two papers dealing with green algal barcoding. The marker we are advocating for Chlorophyta is tufA. The data in support of this choice, primers, etc., are in the first paper on greens in that issue. The…"
I’m involved in, or am interested in the following barcoding initiatives
Marine Barcode of Life (MarBOL)
I’m involved in or interested in the following iBOL Working Groups
WG1.8 Marine Bio-Surveillance, WG1.10 Polar Life
Keywords about my barcoding projects (separate each choice with a comma)
Algae, protist, environmental sample, polar
I’m involved in the following aspects of barcoding:
Fieldwork/specimen collecting, Museum work/specimen curation, Labwork/generating barcode sequences, Management of barcode data, Analysis of barcode data, Using barcode data in taxonomy/systematic biology, Using barcode data for other applications, Organizing/managing barcoding projects
ALGA - Algal Life Global Audit
BoFI - Bay of Fundy Biodiversity Inventory
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