Apologies for late reply...
Fungus and plant DNA together could be problem...but in this case you can used plant specific ITS primer and that would surely solve the problem.
"I am trying to amplify the nuclear ribosomal dna internal transcribed spacers (its1 and its4) from leaves of Cyperaceae using "universal" primers.
But some species is host plant for fungi, So Two species were a molecular mixture as a…"
I have got a curated specimen of Viverridae family. The specimen is dried and kept in the museum for many years. I doubt that it is treated with borric powder and other preservative used in taxidermy. How can we remove the inhibitant from the…"
Try to align your sequences with sequences without frame shift and find out the position where problem lies. Then try to compare with .ab files.
Which software do you use for editing trace files"
"Dear Sreejith, There is no specific kit for such isolation. But you can wash your samples with PBS with continuous shaking at slow speed and you can keep this for overnight also and then try to isolate DNA"
This problem is common if your trace file is not properly corrected, sometimes unknowingly bases are inserted. This leads to frame shift. correct your trace files once again
Dr. Swapnil Gaikwad"
Presently I am using MEGA5 software for analyzing the data obtained from sequencing. But it generates one dendrogram for one locus. Is their any free software available for generating a combined dendrograms using more than one…"
I wanted to know the protocol used to make the master mix. I had obtained the amplicons using the Bamboo DNA and barcode primers. Now by using the components of Big dye Direct cycle sequencing kit how can I make the reaction mix to…"
I’m involved in, or am interested in the following barcoding initiatives
FISH-BOL, All Birds Barcoding Initiative, Marine Barcode of Life (MarBOL), DNA Barcoding Initiative for Conservation, Database Working Group, Data Analysis Working Group, Leading Labs Network
I’m involved in or interested in the following iBOL Working Groups
WG1.4 Human Pathogens and Zoonoses, WG1.5 Agricultural and Forestry Pests and Their Parasitoids, WG1.7 Freshwater Bio-Surveillance, WG2.2 Museum Life, WG2.3 Methodological Innovation, WG2.4 Paleobarcoding
Keywords about my barcoding projects (separate each choice with a comma)
Lepidoptera, Western Ghats, Wolbachia
I’m involved in the following aspects of barcoding:
Fieldwork/specimen collecting, Labwork/generating barcode sequences, Analysis of barcode data, Using barcode data in taxonomy/systematic biology, Using barcode data for other applications
Currently I am doing barcoding of Lepidoptera from Western Ghats along with bugs
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I will shortly be starting barcoding Lepidoptera of Eastern Himalayas. I came across your profile and see that you already are working along the same line in Western Ghats?
Since I too have moved to Pune recently, it makes sense we share some information and keep in touch?
Let me know if thats ok with you.
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