I have got a curated specimen of Viverridae family. The specimen is dried and kept in the museum for many years. I doubt that it is treated with borric powder and other preservative used in taxidermy. How can we remove the inhibitant from the…"
Try to align your sequences with sequences without frame shift and find out the position where problem lies. Then try to compare with .ab files.
Which software do you use for editing trace files"
"Dear Sreejith, There is no specific kit for such isolation. But you can wash your samples with PBS with continuous shaking at slow speed and you can keep this for overnight also and then try to isolate DNA"
This problem is common if your trace file is not properly corrected, sometimes unknowingly bases are inserted. This leads to frame shift. correct your trace files once again
Dr. Swapnil Gaikwad"
Presently I am using MEGA5 software for analyzing the data obtained from sequencing. But it generates one dendrogram for one locus. Is their any free software available for generating a combined dendrograms using more than one…"
I wanted to know the protocol used to make the master mix. I had obtained the amplicons using the Bamboo DNA and barcode primers. Now by using the components of Big dye Direct cycle sequencing kit how can I make the reaction mix to…"
Presently I am working on DNA barcoding in bamboos. I am facing problem in sequencing. Any one can please help me in providing the protocols for sequencing. I have with me 3500 genetic analyzer and Big dye Direct cycle sequencing kit."
I’m involved in, or am interested in the following barcoding initiatives
FISH-BOL, All Birds Barcoding Initiative, Marine Barcode of Life (MarBOL), DNA Barcoding Initiative for Conservation, Database Working Group, Data Analysis Working Group, Leading Labs Network
I’m involved in or interested in the following iBOL Working Groups
WG1.4 Human Pathogens and Zoonoses, WG1.5 Agricultural and Forestry Pests and Their Parasitoids, WG1.7 Freshwater Bio-Surveillance, WG2.2 Museum Life, WG2.3 Methodological Innovation, WG2.4 Paleobarcoding
Keywords about my barcoding projects (separate each choice with a comma)
Lepidoptera, Western Ghats, Wolbachia
I’m involved in the following aspects of barcoding:
Fieldwork/specimen collecting, Labwork/generating barcode sequences, Analysis of barcode data, Using barcode data in taxonomy/systematic biology, Using barcode data for other applications
Currently I am doing barcoding of Lepidoptera from Western Ghats along with bugs
Comment Wall (4 comments)
You need to be a member of Connect.BarcodeofLife.net to add comments!
I will shortly be starting barcoding Lepidoptera of Eastern Himalayas. I came across your profile and see that you already are working along the same line in Western Ghats?
Since I too have moved to Pune recently, it makes sense we share some information and keep in touch?
Let me know if thats ok with you.
Thank you for taking the time to join. Here are a few things that you can do in the community.
After completing your online profile, please add yourself to the member map.
Next, invite your colleagues and search our members for people you know and invite them to be your colleague in the network.
Or browse our Forum and ask a question.
Please join a Group or start your own, as groups are a great way to express your special interest, be it a taxonomic group, a regional or organizational affiliation.
Have a question? Ask one of our hosts.
A few more...
Please contribute to our Blog. Simply choose “Add a Blog Post” under Blogs.
If you already have a blog, you can share it through RSS either on your page or on our rss pages. Just ask me how.
Or create a group and invite colleagues to join. Use “Send Message to Group” to email everyone in your group, start discussions specific to your group’s interests, add widgets and more.
For assistance, don’t hesitate to contact me.
Again, thank you for joining our growing online community. I look forward to connecting with you soon.