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Fiona Nelima Mumoki's Groups
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CBOL Data Analysis Worki…
58 members
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Biocode LIMS plugin
49 members
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ECBOL
37 members
Fiona Nelima Mumoki's Discussions
Complementary bases represented on Electropherogram
1 Reply
Hi everybody,I have this set of sequences belonging to a fragment of nuclear DNA. The thing is, a portion of the sequence seems to have the complementary bases also represented. So, at one position…Continue
Tags: bases, DNA, BioEdit, complementary, editing
Started this discussion. Last reply by Lee Weigt Aug 13, 2012.
DNA extraction from Propolis
8 Replies
Hi everyone,My name is Fiona Mumoki. I am working on Honey bee ecology and health and as part of the project we are looking at honeybee propolis. I have tried DNA extraction for this in vain, maily…Continue
Tags: Reisns, Propolis, Phenolics, Plant, extraction
Started this discussion. Last reply by Fiona Nelima Mumoki Mar 12, 2012.
Fiona Nelima Mumoki's Page
Latest Activity
Fiona Nelima Mumoki posted a discussionComplementary bases represented on Electropherogram
Hi everybody,I have this set of sequences belonging to a fragment of nuclear DNA. The thing is, a portion of the sequence seems to have the complementary bases also represented. So, at one position where you have an A you also get a “shadowed” T, and where you have a C you also get a “shadowed” G. Has anyone ever encountered this? If so, how do you solve this when you are editing sequences?Thank you.See More
Fiona Nelima Mumoki replied to Rasika Bhagwat's discussion 'Data analysis using MEGA' in the group CBOL Data Analysis Working Group (DAWG)"I am interested in hearing what you finally came up with and how you went about it.
For me, First I construct different types of trees using the options available to me then cross them off one by one. I never use NJ especially when its a…"
Fiona Nelima Mumoki replied to C.Vadivalagan's discussion 'software'"It really matters what your intentions were in the first place. What questions do you plan to answer using the data you have obtained?
If you are planning to create BARCODES for the organisms you have collected and worked on, I suggest you…"
Fiona Nelima Mumoki replied to Fiona Nelima Mumoki's discussion 'DNA extraction from Propolis'"Hi Sona,
Sorry about that.
Just one wash should do the trick although you should do it experimentally and adjust if need be.
Let me know how it turns out,
Fiona.
"
sona Jain replied to Fiona Nelima Mumoki's discussion 'DNA extraction from Propolis'"hi Fiona,
I just need to know how many washes with hexane and for how long?
DNA extraction is no problem..
hugs
sona"
Fiona Nelima Mumoki might attend Kris Jett's event
Third European Conference for the Barcode of Life (ECBOL3) at Royal Flemish Academy of Belgium for Sciences and the Arts
September 17, 2012 to September 20, 2012
The European Consortium for the Barcode of Life (ECBOL), the Royal Belgian Institute of Natural Sciences (RBINS) and the Royal Museum for Central Africa (RMCA) invite you to join us for the 3rd ECBOL conference under the theme “Barcoding of Organisms of Policy Concern”. The conference will take place at the Royal Flemish Academy of Belgium for Sciences and the Arts which is located in the historical center of Brussels, next to the Royal Palace and within walking distance (10 min) of the central…See More
Fiona Nelima Mumoki replied to Rasika Bhagwat's discussion 'Data analysis using MEGA' in the group CBOL Data Analysis Working Group (DAWG)"Hmmmm.....good question. Please tell me you solved this problem and what did you conclude?
I am clear on when to use Maximam likelihood, NJ and Minimum evolution options but I am not sure about Kimura 2. Care to share the…"
Fiona Nelima Mumoki joined Steven Stones-Havas's group
Biocode LIMS plugin
The Biocode LIMS plugin is a free suite of plugins to Geneious that allow you to track and manage your entire lab workflow, from tissue samples through to Sequence analysis and Genbank Submission. Visit our website to download the plugins.See More
Fiona Nelima Mumoki joined Lorenzo Lombard's group
ECBOL
For all research groups active in barcode development and/or research in Europe and developing countries in Africa.
Fiona Nelima Mumoki replied to Fiona Nelima Mumoki's discussion 'DNA extraction from Propolis'"Hi Sona,
I have actually never tried it but the hexane is never diluted.
So, the extraction technique is adjusted to get rid of the high wax and reisn content. The rest of the extraction is pretty standard. There are 2 washes with 70%…"
sona Jain replied to Fiona Nelima Mumoki's discussion 'DNA extraction from Propolis'"Hi Fiona
I have hexane so will try with it. 100% hexane or diluted? how many washes and how long? at room temperature?"
Fiona Nelima Mumoki replied to Fiona Nelima Mumoki's discussion 'DNA extraction from Propolis'"Hi Sona,
I sure will!
Best,
Fiona."
sona Jain replied to Fiona Nelima Mumoki's discussion 'DNA extraction from Propolis'"thanks Fiona. if u know anyone working with DNA extraction from propolis with success please let me know....
hugs
sona"
Profile Information
- Institution/Organization
- International Center of Insect Physiology and Ecology (icipe)
- Title/Position
- Other
- If Other Title, please specify
- Graduate student and Research Assistant
- Website
- http://www.icipe.org
- I’m involved in, or am interested in the following barcoding initiatives
- DNA Barcoding Initiative for Conservation, Database Working Group, Data Analysis Working Group
- I’m involved in or interested in the following iBOL Working Groups
- WG1.1 Vertebrates, WG1.2 Land Plants, WG1.3 Fungi, WG1.6 Pollinators, WG2.1 Barcoding Biotas
- Keywords about my barcoding projects (separate each choice with a comma)
- Honeybees
- I’m involved in the following aspects of barcoding:
- Fieldwork/specimen collecting, Labwork/generating barcode sequences, Management of barcode data, Analysis of barcode data, Using barcode data in taxonomy/systematic biology, Using barcode data for other applications, Organizing/managing barcoding projects
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