List of primers
Hi everybody,Does anybody have a list with all the primers sequences aproved for each phyllum?, I am trying to join all this information in one documment. Thanks a lot. DiegoSee More
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Diego Pignataro's Groups
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Barcoding All Lepidoptera
42 members
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Fungi
102 members
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Marine Barcoding
64 members
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Lab Operations
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CBOL Data Analysis Worki…
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Diego Pignataro's Discussions
List of primers
Hi everybody,Does anybody have a list with all the primers sequences aproved for each phyllum?, I am trying to join all this information in one documment. Thanks a lot. DiegoContinue
Started May 20, 2011
DNA from ancient seeds
4 Replies
Hi everybody,My work group and I are trying to get DNA from ancient seeds trying (Solanum, Cucurbita, Lagenaria and Bunchosia samples) which varied in age from 50 - 350 years old or even more. Can…Continue
Tags: samples, old, ancient, seeds, DNA
Started this discussion. Last reply by Diego Pignataro Jan 18, 2011.
Diego Pignataro's Page
Latest Activity
Diego Pignataro replied to John Chenery's discussion 'iBOL Funding News'
Hi,
Who should I contact to get more information about this funding?. We are developing DNA barcodes for medicinal plants in Peru as well for fungus and some lepidopteras. We are very interested in the field and continue with our…
Barcoding All Lepidoptera
Learn more and find out how to join efforts toward assembling a comprehensive DNA barcode library for all species of butterflies and moths
Diego Pignataro joined Rodolphe Rougerie's group
Fungi
To provide up-to-date information on fungal barcoding and to facilitate communication and the development of collaboration.
Diego Pignataro joined Conrad Schoch's group
Diego Pignataro replied to Diego Pignataro's discussion 'DNA from ancient seeds'
Hi Isaac,
Thanks a lot for your commments and advices. Happy to meet you!. Here is my skype account to keep in touch easily: diego.pignataro (peru)
totally right about not to be…
Diego Pignataro replied to Diego Pignataro's discussion 'DNA from ancient seeds'
Hi Dhivya,
I am really sorry for my delayed answer. I haven´t seen this reply before. Give me your skype account to keep in touch more frequently, I do believe that we have lots of experiences to share.
Mine is: diego.pignataro
I…
Isaac Chow replied to Diego Pignataro's discussion 'DNA from ancient seeds'
Hi Diego,
Me again, we have experience in extracting decades bones for forensic DNA. In this case, CTAB usually perform well over the guanidine hcl. Or simply use QIagen investigator kit. further, try to extract the core part of the seed…
Diego Pignataro replied to Tiago Andrade's discussion 'PCR for fungi's COI region (cytochrome oxidase I)'
Hi Isaac,
Thanks a lot for ur replay. Yes, I did..how?..well I performed an alignment with a group of peperomias and search the more conservated region (above 500bp) et voilá. BUT I do have problems with my cinchonas samples..not worked it…
Diego Pignataro replied to Tiago Andrade's discussion 'PCR for fungi's COI region (cytochrome oxidase I)'
I´m agree with Isaac. I will try this primers in my fungus sampels next week. Now we are trying to get an appropiate DNA extraction protocol (basidiomicetos) do you suggest one?
I will try with this:
Rapid DNA Extraction for Screening…
Diego Pignataro replied to Tiago Andrade's discussion 'PCR for fungi's COI region (cytochrome oxidase I)'
I´m agree with Isaac. I will try this primers in my fungus sampels next week. Now we are trying to get an appropiate DNA extraction protocol (basidiomicetos) do you suggest one?
I will try with this:
Rapid DNA Extraction for Screening…
Profile Information
- Institution/Organization
- Cayetano Heredia Univesity
- Title/Position
- Researcher/Faculty member
- I’m involved in, or am interested in the following barcoding initiatives
- Plant Working Group
- I’m involved in or interested in the following iBOL Working Groups
- WG1.2 Land Plants, WG1.10 Polar Life, WG2.2 Museum Life, WG2.3 Methodological Innovation, WG2.4 Paleobarcoding
- Keywords about my barcoding projects (separate each choice with a comma)
- Medicinal plants, cinchona, peperomia, piper, barcode, plants, Peru
- I’m involved in the following aspects of barcoding:
- Labwork/generating barcode sequences, Management of barcode data, Analysis of barcode data
- Current Project(s)
- Development of DNA barcoding for three plant species of medicinal importance in Perú: Cinchona, Piper and Peperomia
Comment Wall (4 comments)
- At 9:36am on May 9, 2010,
Rodolphe Rougerie said… - Hi Diego,
If still in France - near Toulouse - you may want to contact Christophe Thebaud and/or Jerome Chave (Universite Paul Sabatier, Toulouse, lab. Evolution et Diversite Biologique) who are involved in plant barcoding project in French Guiana (see their webpages for contact info at http://www.edb.ups-tlse.fr/equipe1/chave.htm and http://www.edb.ups-tlse.fr/equipe1/Site_Thebaud/Home.html).
Best of luck for your projects in Peru, and enjoy France :-)
- At 3:22pm on December 5, 2009,
Jacqueline Mackenzie-Dodds said… - Hi Diego - thanks for your message. Feel free anytime to contact me or any of us in the Molecular Biology Laboratories at the NHM London. Come and visit us and see our lovely new DC2 building and molecular facilities!
- At 9:56am on November 24, 2009,
Matthew Fisher said… - Hello and welcome!
Thank you for taking the time to join. Here are a few things that you can do in the community.
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Again, thank you for joining our growing online community. I look forward to connecting with you soon.
-Matthew Fisher
- At 9:19am on November 20, 2009,
Megan A. Milton said… - Dear Diego,
Please contact me at mmilton@boldsystems.org and I can assist you with getting your data online.
Cheers,
Megan
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