Connect.BarcodeofLife.net

international online community for dna barcoding professionals

D. ESTHER LEBONAH
  • India
Share

D. ESTHER LEBONAH's Groups

D. ESTHER LEBONAH's Discussions

Barcoded pyrosequencing

Started this discussion. Last reply by D. ESTHER LEBONAH Dec 30, 2013. 2 Replies

Hai to all,               Can Barcoded pyrosequencing identify bacteria found in human habitats at species level? Please reply me anyone.Continue

Barcoded Pyrosequencing

Started this discussion. Last reply by Thomas Horn Dec 12, 2013. 5 Replies

 Hai, anybody please help me to solve my problem.         My problem is               Can I use a universal  primer set ( i.e.,  containing  adaptor sequence, specific bacterial barcode…Continue

The difference between DNA Barcoding and Barcoded pyrosequencing

Started Nov 18, 2013 0 Replies

Hai, I need to know the clear difference between DNA Barcoding and Barcoded pyrosequencing. Please help me anyone.Continue

DNA EXTRACTION

Started this discussion. Last reply by D. ESTHER LEBONAH Nov 25, 2013. 10 Replies

Hai, I need a clear protocol for extraction of each and every bacterial DNA that is present in human milk (without using any kit and with using cost effective chemicals). If I need to amplify 16s…Continue

 

D. ESTHER LEBONAH's Page

Latest Activity

D. ESTHER LEBONAH replied to D. ESTHER LEBONAH's discussion 'Barcoded pyrosequencing'
"Thank you very much sir for your reply. Sorry for the delay to communicate with you.                 Please send your publications on this topic. You said  'species level detection is possible, if I…"
Dec 30, 2013
Prasanna Kumar replied to D. ESTHER LEBONAH's discussion 'Barcoded pyrosequencing'
"Barcoded pyrosequencing can identify bacteria. Many works in Indian perspectives have come recently. IIsc have published bactrial flora of healthy and obese child using barcoded pyrosequencing. I have communicated a manuscript recently on community…"
Dec 21, 2013
D. ESTHER LEBONAH posted a discussion

Barcoded pyrosequencing

Hai to all,               Can Barcoded pyrosequencing identify bacteria found in human habitats at species level? Please reply me anyone.See More
Dec 18, 2013
Thomas Horn replied to D. ESTHER LEBONAH's discussion 'Barcoded Pyrosequencing'
"The same to you! Kind regards Thomas"
Dec 12, 2013
D. ESTHER LEBONAH replied to D. ESTHER LEBONAH's discussion 'Barcoded Pyrosequencing'
"O.K sir, I'll surely try researchgate today. Whenever you get a little knowledge on this topic in future, please suggest me. I wish you all the best for your bright future and I hope you'll surely get success in your work. Best…"
Dec 12, 2013
Thomas Horn replied to D. ESTHER LEBONAH's discussion 'Barcoded Pyrosequencing'
"It seems like you are as alone on this as myself - I am in plants not bacteria. The question if you can use a primer for the bacterial communities you are interested in, can be answered by finding evidence of success in the literature using that…"
Dec 11, 2013
D. ESTHER LEBONAH replied to D. ESTHER LEBONAH's discussion 'Barcoded Pyrosequencing'
"Thank you sir for taking time for me again. Sir, I've followed all of your suggestions but still I'm really struggling because I'm doing this alone for the first time and I have no seniors to help me in this way. I want to…"
Dec 11, 2013
Thomas Horn replied to D. ESTHER LEBONAH's discussion 'Barcoded Pyrosequencing'
"Dear D.Esther Lebonah, I am sorry to see that you are still struggling with that question. Can you answer the following questions: 1. Can you make an estimate to "what kind" of bacteria you will encounter in "particular human…"
Dec 11, 2013
D. ESTHER LEBONAH replied to Mohammad Al-Deeb's discussion 'Second generation Seq vs. Sanger'
"      Sir,             I think, Barcoded Pyrosequencing is better than Sanger sequencing for bacteria, I want to do this type of research for bacterial identification. Please see this link…"
Dec 11, 2013
D. ESTHER LEBONAH posted a discussion

Barcoded Pyrosequencing

 Hai, anybody please help me to solve my problem.         My problem is               Can I use a universal  primer set ( i.e.,  containing  adaptor sequence, specific bacterial barcode sequence) either directly to amplify bacteria present at particular human habitats in normal PCR or  to pyrosequence amplified PCR products ( with conventional universal primers)? . I found that primerset in the publication 'Error-correcting  barcoded primers allow hundreds of samples to be pyrosequenced in…See More
Dec 11, 2013
D. ESTHER LEBONAH replied to D. ESTHER LEBONAH's discussion 'DNA EXTRACTION'
"Thank you sir for taking the time. I'll follow all of your suggestions.                                                …"
Nov 25, 2013
Thomas Horn replied to D. ESTHER LEBONAH's discussion 'DNA EXTRACTION'
"Dear D.E.Lebonah, in my opinion barcoding primers are those which bind to a conserved region in the genome of a wide variety of organisms. They are also called universal barcoding primers. There are some good working primers for the cytochrome c…"
Nov 25, 2013
D. ESTHER LEBONAH replied to D. ESTHER LEBONAH's discussion 'DNA EXTRACTION'
"Respected sir,     So, what is your opinion on using that set of primers in my work?Because they prepared primer set for all the species of bacteria. Using them directly may amplify particular part of 16s rRNA region. But you wrote that…"
Nov 25, 2013
Thomas Horn replied to D. ESTHER LEBONAH's discussion 'DNA EXTRACTION'
"Dear D.E.Lebonah, I think we first should make a distinction between barcoded primers and DNA Barcoding primers. In the publication you mentioned the topic is to tag primers with barcode sequences to increase the number of samples that can be…"
Nov 21, 2013
D. ESTHER LEBONAH replied to D. ESTHER LEBONAH's discussion 'DNA EXTRACTION'
"Thank you sir for providing the requested information and for your consideration. Sir, if you have time, please clear me this doubt also.                       I don't know about using…"
Nov 21, 2013
Thomas Horn replied to D. ESTHER LEBONAH's discussion 'DNA EXTRACTION'
"It seems like you do not need to clone the different subpopulations of 16s rDNA because NGS pyrosequencing can handle those. In my line of work - with plants - we usually need to isolate the respective DNA from one plant at a time, because we are…"
Nov 20, 2013

Profile Information

Institution/Organization
S.V UNIVERSITY
Title/Position
Doctoral student
If Other Title, please specify
DNA BARCODING ON BACTERIA

My Comment Wall

You need to be a member of Connect.BarcodeofLife.net to add comments!

Join Connect.BarcodeofLife.net

  • No comments yet!
 
 
 

Translate

Tory's site-wide code

New to the Connect network?


Watch our Intro Webinar


Introduce yourself to the Connect community


Write a blog post


Ask a question

Tory's code

© 2017   Created by Mike Trizna.   Powered by

Badges  |  Report an Issue  |  Terms of Service