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Bryn T. M. Dentinger's Page
Latest Activity
Bryn T. M. Dentinger replied to Keith Seifert's discussion 'Defining the boundaries of the ITS barcode' in the group Fungi"right, thanks for pointing out my taxonomic bias! looks like there is no universally constant region within the 28S that could serve as a handle without needing exceptions.
but rather than trimming sequences to within these motifs, what would be…"
Bryn T. M. Dentinger replied to Keith Seifert's discussion 'Defining the boundaries of the ITS barcode' in the group Fungi"we have been routinely trimming our ITS sequences to beginning and ending with the CATTA- and -GACCT motifs. The -GACCT motif can vary a bit depending on which groups you work in (highly variable in Hygrocybe, for instance), but in my experience…"
Bryn T. M. Dentinger replied to Conrad Schoch's discussion 'Comment from Bryn Dentinger' in the group Fungi"Hi Lore,
We have not used these new primers in lichens and very few ascos. Since they were designed with basidios in mind (Agaricomycetes, in fact) I cannot recommend them over the standard ITS4 and ITS5 pair that is typically used for ascos.
bryn"
Bryn T. M. Dentinger joined Conrad Schoch's group
Fungi
To provide up-to-date information on fungal barcoding and to facilitate communication and the development of collaboration.
Profile Information
- Institution/Organization
- Royal Botanic Gardens, Kew
- Title/Position
- Researcher/Faculty member
- I’m involved in, or am interested in the following barcoding initiatives
- DNA Barcoding Initiative for Conservation
- I’m involved in or interested in the following iBOL Working Groups
- WG1.3 Fungi
- I’m involved in the following aspects of barcoding:
- Fieldwork/specimen collecting, Museum work/specimen curation, Labwork/generating barcode sequences, Management of barcode data, Analysis of barcode data, Using barcode data in taxonomy/systematic biology, Organizing/managing barcoding projects
Comment Wall (1 comment)
- At 9:11am on October 4, 2010,
Matthew Sileo said… - Hello and welcome!
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