"we are not working on herbarium samples etc., but with very low template concnetrations and sometimes meaterial with inhibitors. We often use BSA, and what helps a lot is also a robust PCR system - we use the Phusion polymerase, as a mastermix…"
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Arthur Schuessler
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Arthur Schuessler's Groups
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Environmental Barcoding
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Field Operations
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Fungi
106 members
Arthur Schuessler's Discussions
'Glomus intraradices DAOM197198', a model fungus in arbuscular mycorrhiza research, is not Glomus intraradices
1 Reply
Stockinger H, Walker C, Schüßler A (2009)'Glomus intraradices DAOM197198', a model fungus in arbuscular…Continue
Tags: obligate, biotrophs, AM, fungi, arbuscular
Started this discussion. Last reply by Conrad Schoch Nov 3, 2010.
DNA-based species-level detection of arbuscular mycorrhizal fungi: one PCR primer set for all AMF.
Krüger M, Stockinger H, Krüger C, Schüßler A (2009)New Phytologist 183: 212-223 […Continue
Tags: obligate, biotrophs, AM, fungi, arbuscular
Started Oct 23, 2010
DNA barcoding of arbuscular mycorrhizal fungi
Stockinger H, Krüger M, Schüßler A (2010)New Phytologist 187: 461-474 […Continue
Tags: obligate, biotrophs, AM, fungi, arbuscular
Started Oct 23, 2010
Arthur Schuessler's Page
Latest Activity
Arthur Schuessler replied to Conrad Schoch's discussion 'Problems with ITS' in the group Fungi"yes, we will submit longer sequences anyway. but it may be that identification tools will base on ITS only, and then people may tend to produce ITS only. The dimension of problem is a bit different between Glomeros and Ascos, in Glomeros it is a…"
Arthur Schuessler replied to Conrad Schoch's discussion 'Problems with ITS' in the group Fungi"Hello all,
as communicated we can not resolve close relatives for Glomeromycota, if using the ITS region only. Large intraspecific variability, and I doubt that any method can reliably resolve the species based on the ITS region only (other…"
Arthur Schuessler replied to Conrad Schoch's discussion 'Problems with ITS' in the group Fungi"ITS region (ITS1+5.8S+ITS2) ALONE does not work for Glomeromycota, for close relatives, from all we know. several publications show or indicate that, already"
Arthur Schuessler replied to Conrad Schoch's discussion 'RPB1 and 2 problems' in the group Fungi"just another short note - regards "orthologues" - when I once tried to get RPB2 for Geosiphon (in Archaeosporales, an ancestral lineage in Glomeromycota), I got (beside dozens of other things), after a while of trying an RPC2 subunit (RNA…"
Arthur Schuessler replied to Conrad Schoch's discussion 'RPB1 and 2 problems' in the group Fungi"Just to note, we also have problems with RPBs for the Glomeromycota. RPB2 for the ancestral Glomero-lineages we gave up already 2006 (AFTOL). Recent RPB1 PCRs using the recommended degenerate forward primer and rev. primers recommended by Dirk…"
Arthur Schuessler replied to Henrik Nilsson's discussion 'Mortierella exposed' in the group Fungi"We are defining some epitypes for the Glomeromycota. Several types do not "exist" or material is mixed, or degraded - so these species are somehow "dead". They can be "redefined" by careful selection of epitypes, that…"
Arthur Schuessler replied to Conrad Schoch's discussion 'Lack of gene variation' in the group Fungi"further Glomeromycota SSU data of Glomus Group Ab (includes the fast growing Glomeromycs) recently were published, we are in progress of making some more sequences, too. So, enough data should be available.
Arthur"
Arthur Schuessler replied to Conrad Schoch's discussion 'Additional primers?' in the group Fungi"OK, this is 100 bp more (3') than our "AMF specific" (I write like this because no primer set is sure to be 100% specific) nested primers, and >100 bp less (3') than our outer primers. compatible regions, fits"
Conrad Schoch replied to Arthur Schuessler's discussion ''Glomus intraradices DAOM197198', a model fungus in arbuscular mycorrhiza research, is not Glomus intraradices'"Hi Arthur, this may be important for the genome resources people at GenBank to know too. I will forward it. Did you get any response from parties concerned?"
Arthur Schuessler replied to Conrad Schoch's discussion 'Additional primers?' in the group Fungi"how long will the target region in the 5' LSU be, if included?"
Arthur Schuessler replied to Conrad Schoch's discussion 'Additional primers?' in the group Fungi"our primers we use for Glomeromycota 200bp SSU+ITS region+800 bp LSU (1.8 kb or 1.5 kb in nested PCR) are in Krüger M, Stockinger H, Krüger C, Schüßler A (2009)
DNA-based species-level detection of arbuscular mycorrhizal fungi:…"
Arthur Schuessler replied to Conrad Schoch's discussion 'Lack of gene variation' in the group Fungi"we have SSU data - I will send you a draft of a paper in 3-4 weeks where you can get an idea abou the data. it is just that we do not intend to sequence many variants, as one can rel. easily show that species borders cannot be resolved…"
Arthur Schuessler replied to Conrad Schoch's discussion 'Lack of gene variation' in the group Fungi"In our case (Glomeromycota = arbuscular mycorrhizal fungi), the SSU shows intraspecific and intrasporal variability (several variants in one fungal asexual, multinucleated spore). But we use the SSU for robust phylogenetic analysis - in combination…"
Arthur Schuessler commented on Kris Jett's group 'Field Operations'"Dear all, we are also sampling (plant roots with colonizing fungi) and directly storing them in 80% EtOH. We later store at -20°C, also in 80% EtOH. Is there a comparison between storage in EtOH and DMSO? Differences in DNA extraction…"
Profile Information
- Institution/Organization
- LMU Munich
- Title/Position
- Researcher/Faculty member
- Website
- http://www.amf-phylogeny.com
- I’m involved in, or am interested in the following barcoding initiatives
- DNA Barcoding Initiative for Conservation, Plant Working Group, Database Working Group, Data Analysis Working Group, Leading Labs Network
- I’m involved in or interested in the following iBOL Working Groups
- WG1.3 Fungi, WG1.9 Terrestrial Bio-Surveillance, WG2.1 Barcoding Biotas, WG2.3 Methodological Innovation, WG2.4 Paleobarcoding
- Keywords about my barcoding projects (separate each choice with a comma)
- Fungi, Glomeromycota, arbuscular mycorrhizal fungi, plant symbionts
- I’m involved in the following aspects of barcoding:
- Fieldwork/specimen collecting, Labwork/generating barcode sequences, Management of barcode data, Analysis of barcode data, Using barcode data in taxonomy/systematic biology, Using barcode data for other applications, Organizing/managing barcoding projects
- Current Project(s)
- DNA barcoding of Glomeromycota,
AM fungi from South Ecuador,
454 Titanium pyrosequencing for barcode-based monitoring of Glomeromycota,
Comment Wall (1 comment)
- At 9:31am on September 27, 2010,
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