"Great discussion! I agree with Bevan regarding the wording of (d) in that you are referring to "multiple sequence alignments" and not "multiple trace file alignments", the latter of which is a mandatory requirement for any…"
I am posting a question from Jose Dianese as he is having some issues with his fungi. My guess is not enough DNA for the PCR to amplify a product. Any thoughts?
I am having hard time…"
"Good thoughts Keith.
My lab edits all ITS sequences to include the 5' and 3' primers. In most cases, this is ITS1F and ITS4 and would include your two proposed motifs above so I'm good with this.
If I'm not…"
"We have also been using:
Mcm7-1447rev: CAT IAC IGC IGC IGT RAG RCC
in our lab, but with limited success. The primers Mcm7-709for and Mcm7-1348rev seem to work pretty well for most pyrenos, loculos and discos. We have had up to 80% success trying…"
I’m involved in the following aspects of barcoding:
Fieldwork/specimen collecting, Museum work/specimen curation, Labwork/generating barcode sequences, Management of barcode data, Analysis of barcode data, Using barcode data in taxonomy/systematic biology, Organizing/managing barcoding projects
Barcoding the ascomycetes of the Great Smoky Mountains National Park
Barcoding the Neotropical ascomycetes
Barcoding the freshwater ascomycetes
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