"Dear All,
I am posting a question from Jose Dianese as he is having some issues with his fungi. My guess is not enough DNA for the PCR to amplify a product. Any thoughts?
Andy
Dear Andy,
I am having hard time…"
international online community for dna barcoding professionals
-
Andrew Miller
- Male
- Champaign, IL
- United States
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Andrew Miller's Page
Latest Activity
Andrew Miller replied to Keith Seifert's discussion 'Defining the boundaries of the ITS barcode' in the group Fungi"Good thoughts Keith.
My lab edits all ITS sequences to include the 5' and 3' primers. In most cases, this is ITS1F and ITS4 and would include your two proposed motifs above so I'm good with this.
If I'm not…"
Andrew Miller replied to Conrad Schoch's discussion 'RPB1 and 2 problems' in the group Fungi"Anyone know how long the PCR product of RPB1 should be in pyrenos or loculos?
Thanks,
Andy
"
Andrew Miller replied to Pradeep Kumar Divakar's discussion 'PCR protocol' in the group Fungi"We use 1.0µL of each 10mM primer in our 25µL PCR reactions.
Andy"
Andrew Miller replied to Pradeep Kumar Divakar's discussion 'PCR protocol' in the group Fungi"We have also been using:
Mcm7-1447rev: CAT IAC IGC IGC IGT RAG RCC
in our lab, but with limited success. The primers Mcm7-709for and Mcm7-1348rev seem to work pretty well for most pyrenos, loculos and discos. We have had up to 80% success trying…"
Andrew Miller joined Conrad Schoch's group
Fungi
To provide up-to-date information on fungal barcoding and to facilitate communication and the development of collaboration.
Profile Information
- Institution/Organization
- Illinois Natural History Survey
- Title/Position
- Researcher/Faculty member
- I’m involved in or interested in the following iBOL Working Groups
- WG1.3 Fungi
- Keywords about my barcoding projects (separate each choice with a comma)
- Fungi, Ascomycota, ascomycetes, dothideomycetes, pyrenomycetes
- I’m involved in the following aspects of barcoding:
- Fieldwork/specimen collecting, Museum work/specimen curation, Labwork/generating barcode sequences, Management of barcode data, Analysis of barcode data, Using barcode data in taxonomy/systematic biology, Organizing/managing barcoding projects
- Current Project(s)
- Barcoding the ascomycetes of the Great Smoky Mountains National Park
Barcoding the Neotropical ascomycetes
Barcoding the freshwater ascomycetes
Comment Wall (1 comment)
- At 9:11am on October 4, 2010,
Matthew Sileo said… - Hello and welcome!
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-Matt Sileo
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