Sponge Barcoding

Comment

Comment by Giulia Fassio on March 4, 2011 at 1:05pm

Thank you for the explanation and for the help! I will start checking in literature for different primer.

Giulia

Comment by Sergio Vargas R. on March 4, 2011 at 4:07am

????

by Folmer fragment I mean the standard barcoding fragment.

sergio

Comment by Giulia Fassio on March 4, 2011 at 3:11am

Hi Sergio,

what do you mean for "Folmer" fragment? I need to amplify just COI gene.

Good idea, discussing off list could be easier

Giulia 

Comment by Sergio Vargas R. on March 2, 2011 at 3:59pm

Hi Giulia,

if you want to barcode your 5 sponge, you'r kind of stucked to the "Folmer" fragment. If you want to do something else, well you could check the literature (?) Sponge specific primers for the Folmer region are rather hard to find, I must say.

If you want we can disscuss your Mycale problems off list to avoid long postings.

sergio

Comment by Giulia Fassio on March 2, 2011 at 12:55pm

Hi Sergio,

do you know if there is some other kind of primer maybe specific for sponges? Because design specific one for my 5 samples could be too expensive considering my project.

Giulia

Comment by Sergio Vargas R. on March 1, 2011 at 6:14am

Hi Giulia,

I'm also working with Antarctic sponges. You should try to desing more specific primers. If you are only working with Mycale this should not be a problem.

sergio

Comment by Giulia Fassio on March 1, 2011 at 5:57am

Hello, I am trying to extract and amplify 5 specimens of Mycale conserved in ethanol since 2004, sampled in Antarctica. I have tried with generic primers for COI (HCO LCO) and with a phenol-cloroformium protocol for extraction but until now I have found only a bryozoan... any suggestion?

Thanks :)

Giulia

Comment by Gert Woerheide on February 24, 2011 at 4:22pm

Today two new reference records of Halisarca species from Ereskovsky et al. 2001 Zootaxa were released at the Sponge Barcoding Database (www.spongebarcoding.org).

Comment by Gert Woerheide on February 5, 2011 at 8:59am

Can we move cnidarian specific issues/discussions to a new (cnidarian) group please and keep it spongy here?

Comment by Sergio Vargas R. on February 5, 2011 at 7:26am

Hey there,

 

Cat McFadden was also interested in establishing an octocoral barcoding project, porting the sponge barcoding interface. A cnidarian barcoding project might be easier.

 

Frederic, what marker do you guys use for barcoding zoanthids and how specific is it?

 

• ‹ Previous
• 1
• 2
• Next ›
• Page