international online community for dna barcoding professionals
A group related to the barcoding of sponges.
Location: Munich Germany
Latest Activity: Nov 22, 2015
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Thank you for the explanation and for the help! I will start checking in literature for different primer.
by Folmer fragment I mean the standard barcoding fragment.
what do you mean for "Folmer" fragment? I need to amplify just COI gene.
Good idea, discussing off list could be easier
if you want to barcode your 5 sponge, you'r kind of stucked to the "Folmer" fragment. If you want to do something else, well you could check the literature (?) Sponge specific primers for the Folmer region are rather hard to find, I must say.
If you want we can disscuss your Mycale problems off list to avoid long postings.
do you know if there is some other kind of primer maybe specific for sponges? Because design specific one for my 5 samples could be too expensive considering my project.
I'm also working with Antarctic sponges. You should try to desing more specific primers. If you are only working with Mycale this should not be a problem.
Hello, I am trying to extract and amplify 5 specimens of Mycale conserved in ethanol since 2004, sampled in Antarctica. I have tried with generic primers for COI (HCO LCO) and with a phenol-cloroformium protocol for extraction but until now I have found only a bryozoan... any suggestion?
Cat McFadden was also interested in establishing an octocoral barcoding project, porting the sponge barcoding interface. A cnidarian barcoding project might be easier.
Frederic, what marker do you guys use for barcoding zoanthids and how specific is it?
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