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Problems in DNA barcoding

Dear all, This group is intend to solve problems occurred while analyzing barcode sequences and using appropriate method of analysis.

Website: http://problemsindnabarcoding
Location: Pune, Maharashtra, India
Members: 85
Latest Activity: 4 hours ago

Discussion Forum

Sequence error 6 Replies

I have doing my deposition of sequence in the NCBI, but when one of the sequence is deposited I have got a message stating that some or all of the sequences contain reading frame shifts…Continue

Started by sreejith kanholi. Last reply by Gaikwad Swapnil Sopan 4 hours ago.

DNA Barcoding in bacteria 2 Replies

Hai to all, I need 100% details on DNA Barcoding of bacteria from soil samples by culture independent methods. I have a problem from the beginning of bacterial DNA extraction to Pyrosequencing. So,…Continue

Started by D. ESTHER LEBONAH. Last reply by D. ESTHER LEBONAH Nov 20, 2013.

MatK gene region in angiosperm 5 Replies

Dear all,I am currently working on DNA Barcoding of some plants (belonging to Angiosperm). Fistly ı did not use DMSO for MatK gene region. But sequence results are not good and then ı use the DMSO,…Continue

Started by Handan. Last reply by Paula May 29, 2012.

primer 5 Replies

Hello sir/madam,                     I am currently working on  DNA Barcoding of the odonata.Am in the initial stages of sample collection and only limited referece are available on this area of…Continue

Tags: gene, CO1, odonata, primer

Started by Dr.C.VADIVALAGAN. Last reply by Anoja Kurian Jan 6, 2012.

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Comment by Gaikwad Swapnil Sopan 6 hours ago

Dear Sreejith,
There is no specific kit for such isolation. But you can wash your samples with PBS with continuous shaking at slow speed and you can keep this for overnight also and then try to isolate DNA

Comment by sreejith kanholi 6 hours ago

Dear all, I am trying to isolate DNA from Borric acid/sodium borate preserved dried specimen. Is there any isolation Kit available for this type .

Comment by Gaikwad Swapnil Sopan 7 hours ago

Dear Amrita,

This is regarding dendogram, you can concatenate sequence of both the locus and then generate dendogram using MEGA

Comment by Amrita Sinha 8 hours ago

Hello everyone, 

Presently I am using MEGA5 software for analyzing the data obtained from sequencing. But it generates one dendrogram for one locus. Is their any free software available for generating a combined dendrograms using more than one locus.

Thank you.

Comment by Amrita Sinha 8 hours ago

Hello Mam,

I wanted to know the protocol used to make the master mix. I had obtained the amplicons using the Bamboo DNA and barcode primers. Now by using the components of Big dye Direct cycle sequencing kit how can I make the reaction mix to amplify for subjection to sequencing and what procedure must I follow

Comment by PRIYANKA MISHRA yesterday

hello Amrita,

Please elaborate the problems step wise, you are facing during sequencing.

Comment by Amrita Sinha yesterday

Hi,

Presently I am working on DNA barcoding in bamboos. I am facing problem in sequencing. Any one can please help me in providing the protocols for sequencing. I have with me 3500 genetic analyzer and Big dye Direct cycle sequencing kit.

Comment by rehab refaat mohammed on January 26, 2014 at 2:15am

Dear all

i need informations on DNA barcoding of reptiles, please i need help

thanks

Comment by Amir Bashir on November 13, 2013 at 12:41am

Dear all,

me a new worker on barcode of fishes , i request all to send protocals

Comment by Dr.C.VADIVALAGAN on May 8, 2012 at 1:19pm

Dear all,

        I Have completed my molecular Biology work now am planing to move on computation work (Barcoding) now i done molecular Phylogeny and protein Docking but i didnt get any idea about coding software. 

can you sagest coding software, its very useful for completing my research.

Thanking you all

with regards,

Chithravel Vadivalagan

 

 

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