international online community for dna barcoding professionals
New primers and protocols for matK DNA barcoding
These pdfs detail clade-specific primer sets & protocols for the matK barcode region in land plants. These protocols were developed by Alan Forrest, in collaboration with Luke Dunning, Aron Fazekas, Lianming Gao, Sean Graham, Mehrdad Hajibabaei, Pete Hollingsworth, Maria Kuzmina and Damon Little, and the work was funded by the Gordon and Betty Moore Foundation,
If you use these primers please send an email to barcoding@rbge.ac.uk. This is to enable us to provide protocol updates and to solicit feedback on how well the primers perform.
Angiosperm_matK_RBGE_PROTOCOLv1.0.pdf
Gymnosperm_matK_RBGE_PROTOCOLv1.0.pdf
Hornwort_matK_RBGE_PROTOCOLv1.0.pdf
Fern_matK_RBGE_PROTOCOLv1.0.pdf
Liverwort_matK_RBGE_PROTOCOLv.1.1.pdf
Moss_matK_RBGE_PROTOCOLv1.0.pdf
The folder below contains the matK sequence alignments which were used for primer design. These alignments are useful for designing further taxon-specific primers and also for assessing levels of primer mismatch
Hiwhat primers sequence set may I use PCR conditions DNA extraction protocols Need yours healpZafar Continue
Started by Muhammad Zafar Saleem Feb 26.
Please use this space to discuss primers and protocols. To start things off, here are the most up-to-date…Continue
Started by Karen James. Last reply by Anoop.B.S. Mar 14, 2012.
I'm investigating phylogenetic in Panax genus (Araliaceae). I tried some published PCR condition for amplification matK gene with pair of specific primers trnK3914F (5’-TGG GTT GCT AAC TCA ATG G-3’)…Continue
Started by Le Ngoc Trieu Mar 6, 2012.
There were several papers on barcoding medicinal plant given at the last two international barcoding conferences in Taipei and Mexico City. I'm wondering if there is potential for regional or maybe…Continue
Started by David E. Schindel. Last reply by Robab Ghahramanzadeh Oct 25, 2011.
Comment
Comment by JOHN OLAYINKA ATOYEBI on May 14, 2012 at 1:00pm Dear Colleagues, Its good to rejoin this group once again after sometime of absence, since CBOL conference in Taiwan. Looking forward to a good networking and collaboration.
JOHN
Comment by Pete Hollingsworth on March 23, 2012 at 12:36pm matK sequence alignments added to the Connect 'Plants' group front page. These are helpful for primer design and assessing levels of mismatch between primers and a given taxonomic group
Comment by Pete Hollingsworth on February 21, 2012 at 8:43am Some primers and protocols for matK DNA barcoding in land plants are now posted on the 'Plants' group page
Comment by rohini khanapurkar on December 17, 2011 at 1:38pm i also wanted to knw wt shud be the appropriate sample size to barcode a particular plant species.
Comment by rohini khanapurkar on December 17, 2011 at 1:27pm hello all,
I wanted to ask if ITS2 alone can be used as barcoding region for medicinal plants, or atleast can it be used as an identifying marker for medicinal plants, please reply as soon as possible,thanking you.
Comment by Angelia Yulita on November 13, 2011 at 3:41am Hello all !
I'm coming from Indonesia and now is proposed to barcoding some tree species in java island.
I am a newbie !! So please you guys humbly help me with support, discussion and any information about plant barcoding.
Nice to see you guys !!
Best regards,
Angelia.
Comment by Jivan Shakya on November 4, 2011 at 6:19am Hello all,
I am from Nepal and currently undertaking the pilot project on barcoding medicinal plants of Nepal. The pilot project aims to barcode Asparagus racemosus, Dactylorhiza hatagirea and Choerospondias axillaris on the basis of rbcL, matK, psbA_trnH and ITS2 seqences.
I am new to barcoding and have been getting some basics from the literatures. I look forward to getting valuable support from the group members in terms of knowldege sharing.
Regards
Jivan Shakya
Comment by Dhivya on November 1, 2010 at 12:08pm
Comment by Pradeep Kumar Divakar on October 1, 2010 at 10:22am
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