Plants

Comment

Comment by Paula on August 27, 2010 at 2:11am

Hi Olivier and everybody
I am from the University of the Free State, Bloemfontein, South Africa. We have been busy for a few year sequencing plants from the Hyacinthaceae and Amaryllidaceae in South Africa, but started only recently implementing those sequences for Barcoding.

Olivier, we must make contact some time. I met Michelle about two weeks ago when she presented a talk for us. As I said, we work on the above families, and especially the genera Clivia and Lachenalia, but will expad in these families with time. I hope our work does not overlap with your work...

My e-mail address is spiesp@ufs.ac.za

Kind regards,
Paula

Comment by Shoupu He on August 26, 2010 at 8:43pm

Hi all!
I work on cotton in China. I'm very interested in DNA barcoding because we have abundant cotton germplasm. I hope this approach could work on cotton.

Best regards

Dennis He

Comment by Olivier Maurin on August 21, 2010 at 4:23am

Hello all, I work at the University of Johannesburg with Michelle van der Bank, and we wanted to share with you the latest development in regards to plant DNA barcoding in South Africa. Best wishes to all. Olivier
Please feel free to visit and comment on:
http://www.toyotaoutreach.com/

Comment by Diego Pignataro on March 2, 2010 at 4:02pm

hi everybody!

I am in France until the end of May, if there is anybody who works with plant barcoding I would like to visit the lab and share experiences, or if somebody know about one laboratory near Toulouse will be perfect!

Best regards for everybody

Diego

Comment by Hemant Kawalkar on December 23, 2009 at 7:18am

Hi, It feels great to look at the response to Diego's question. I have planned to barcode Indian Drimia species. I am new to DNA barcoding and look forward to get help form this group if at all i face a problem in my work.... Hope every thing goes well.... thanks Karen for making this group!! :)

Comment by Diego Pignataro on December 2, 2009 at 1:54pm

Thanks a lot for all comments and advices!!, hope everything work well for all of you!.

Keep in touch.

Best regards for all,

Diego

Comment by Nadeesha Lewke Bandara on December 2, 2009 at 9:57am

Hi everyone,
about diego;s problem- If you have access to ABI SEQUENCER MACHINE you can do it by your self.IF you have PCR products, before sending to the sequencer you should puriefy PCR products.protocols use for purification depends on the lab.I'm doing matk sequencing for genus Onobrychis using ABI 3730 machine.but my lab not doing commercial sequencing.
Cheers.

Comment by Rasika Bhagwat on December 2, 2009 at 8:52am

Hi
I am Rasika, working at NCL in India. I am working on plant DNA barcoding.

Comment by Luz Ruiz Maqueda on November 25, 2009 at 2:47pm

Hi everyone!
About Diego's question, I sent to Macrogen because it was cheaper from other options... about the programs to align, there are many options to align, all of them are online and they are free of charge, like bioedit (windows plataform, http://www.mbio.ncsu.edu/BioEdit/bioedit.html), clc sequence viewer (for the three plataforms: windows, mac and linux, http://www.clcbio.com/index.php?id=28). If you have money to buy software, I recomend you DNAstar.
Hope this is useful to you.
Cheers!

Comment by Karen James on November 24, 2009 at 1:00pm

Thanks for joining, Diego. Glad to hear you're starting a new project. I suggest starting with the current protocols (pdf) provided on the CBOL Plant Working Group page. At the PWG pre-conference workshop we were told that's where all the up to date info is.

David's right - you'd send your PCR products to your organisation's sequencing lab, or if you don't have one, you should contact the folks at Guelph (e.g. Mehrdad Hajibabaei) and see if they might be able to sequence your products for you.

Most of us are either only just beginning or about to begin working with plant barcodes in BOLD so we'll have to see how that goes!

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