international online community for dna barcoding professionals
New to barcoding - need help with data analysis
My colleage and I are new to DNA barcoding and we need help with data analysis. We have sequenced matK, rbcL, and trnH-psbA in six tree species, and have used this data to determine the presence of a barcode gap. But now we are stuck! We are unsure how to proceed - analyses to perform, including statistical analyses. Any help would be greatly appreciated!
Thanks in advance!
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Replies to This Discussion
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Permalink Reply by Vijaykumar Yogesh Muley on -
Here are few tips,
use these sequences to fish out similar sequences (homologs) from NCBI using BLAST tool. Search against Plant database or taxanomy group. then select homologs that are useful for you analysis and get sequences. Do multiple sequence alignment using ClustalW. It generates guide tree as well. Its not exactly phylogenetic tree but provide rough idea about evolution of your sequences. If you are new to these tools, you can search them in google and then go through documentation on how to use them
i hope this will help you
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