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Can anybody suggest me a good protocol for gDNA isolation from herbarium samples which are 50-75 yrs old and specimen were  were chemically treated with HgCl2

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Hi Sribash: this is going to be very difficult he DNA is likely to be very degraded as the specimens are old and they were treated with Hg. I think that Qiagen make some kits than are supposed to help with difficult samples like these (they were designed for forensics) that you might try, but i have not tried them myself. good luck! Gill



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