Mosquito Barcoding Initiative

Comment

Comment by Rita Zomuanpuii on August 2, 2011 at 4:32am

Currently working on barcoding of Anopheles species of Mizoram, India

Comment by Matthew Bowser on January 7, 2011 at 1:27pm

Monitoring of Culicidae and other terrestrial arthropod taxa using next-gen (environmental sampling) DNA barcoding methods is the long-term objective of a mini-bioblitz we are planning for this summer.  This field season, we will be sampling Culicidae as well as other groups, obtaining determinations on voucher specimens (for which I am seeking collaboration), and submitting these specimens to a DNA barcoding lab to build a regional DNA barcode library.

Comment by Richard Wilkerson on January 7, 2011 at 11:47am

Are you looking for collection and archiving methods?  Basic taxonomy to anchor your sequences is quite important for validating your results.  If so, I can point you to a summary of field and laboratory methods.

Do you have a "nex-gen" method on the horizon? I too am interested in this, especially for Anopheles.

Comment by Matthew Bowser on January 6, 2011 at 7:16pm

I am looking for help with inventorying and obtaining DNAbarcodes of mosquitoes from the Kenai National Wildlife Refuge thissummer.  This is part of a larger arthropod inventory initiative on therefuge, but the Culicidae is a priority group because of their abundance, ecologicalimportance, and prevalence in samples we obtained for our long-term monitoringefforts.  We are building a referencelibrary of DNA barcodes for the refuge to set the stage for monitoring usingnext-generation DNA barcoding methods.

Comment by sambashiva daravath on June 16, 2010 at 12:02am

is there any single nucleotide polymorphism found in mosquito bar codes

Comment by sambashiva daravath on June 15, 2010 at 11:51pm

respected members am working on molecular phylogenetic analysis of mosquitoes. i would like to work along with you.

Comment by T. Ryan Gregory on November 14, 2009 at 3:33pm

Anyone interested in mosquitoes should also consider joining the HealthBOL group. :-)

Comment by Yvonne Linton on November 12, 2009 at 11:16am

On the opening day of the Third international Barcoding Conference, Mexico, Dr Yvonne Linton, on behalf of the MBI consortium presented barcode data for 1900 specimens, corresponding to some 174 recognised mosquito species. Significantly, some 46 cryptic taxa were additionally recovered using DNA barcodes, indicating that mosquito biodiversity make currently be under-represented by some 26%. Introgression, as evidenced by additional ITS2 sequencing was noted in four species pairs. Total data presented included 35% of known species of Anopheles, which includes 90% of reported malaria vectors world-wide.

In addition, Dr Pradeep Kumar gave an excellent presentation on Indian species, to add to his previously published paper on barcoding Indian taxa (link to Kumar et al., 2007) - we invite Dr Kumar to give us an summary of his data.Kumar et al. 2007.pdf