international online community for dna barcoding professionals
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Come share protocols and troubleshooting experiences, compare techniques, and discuss collaborative efforts.
Members: 26
Latest Activity: Sep 2
Discussion Forum
Sharing Lab Protocols 5 Replies
One of the biggest goals of the Community Network is to share information among barcoders, and information about lab protocols is a very high priority. If you have documents that describe the protoco…
Started by David E. Schindel. Last reply by Thomas Horn Sep 2.
THE BEST PROCESSING METHODS FOR NEMATODE PARASITES OF FISH FOR DNA BARCODING ACTIVITY 2 Replies
Dear colleagues, Could anyone assist with the best method for the collection, preservation and fixing of nematode parasites of fish samples for DNA barcoding activity?
Started by MOHAMMAD-MONZOOR. Last reply by MOHAMMAD-MONZOOR Aug 3.
sequencing slightly degraded DNA 2 Replies
Hello, If one amplifies CO1 in two parts with interior primers. Would it be possible to sequence the two parts together in one step? I am asking because we assembling a plate with PCR products of 7…
Started by Patricia Escalante. Last reply by Patricia Escalante Jan 13.
Lab Operations
Having trouble signing in and joining up - 2nd attempt!
Started by Jacqueline Mackenzie-Dodds Dec. 28, 2009.
My Comment Wall
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Comment by Thomas Horn on August 28, 2010 at 4:01pm -
Hi, i am currently confronted with very weak matK amplification of some species. We use dried material to extract DNA from. I am still not convinced that the extraction is the problem.
Did your group solve their problems?
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Comment by Diego Pignataro on March 2, 2010 at 5:11pm
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Hi!
My group is having some problems with matK amplification for fresh and herbarium samples, anybody have a recommendation or a protocol to follow? to improve the PCR reaction?
thanks a lot
Diego
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