problem with protocol

Replies to This Discussion

Permalink Reply by Lee Weigt on December 11, 2009 at 2:01pm

protocols for which steps?
Specimen acquisition
Tissue isolation
Tissue disruption
DNA extraction
Primers/locus
PCR
sequencing

?
Lee

Permalink Reply by sujeet kumar singh on December 11, 2009 at 2:15pm

DNA Isolation and purification...

Permalink Reply by Lee Weigt on December 14, 2009 at 8:51am

which methods were you using? did you have controls to know that is was the methods as opposed to the reagents, plant specimens, etc?
Our standard plant extraction methods are CTAB and Qiagen Plant DNEasy
Lee

Permalink Reply by sujeet kumar singh on December 14, 2009 at 10:21pm

Doyle & Doyle method....... ie CTAB only....

Permalink Reply by Lee Weigt on December 15, 2009 at 9:27am

and you have positive controls? I'll dig up a version of our CTAB adn send it -
Lee

Permalink Reply by Lee Weigt on December 17, 2009 at 9:43am

here is the plant CTAB protocol from our plant group.
I have another that is robot specific for our Autogen DNA extractor, let me know if you have specific interest in that one - likely not.
LEe

Attachments:

• Plant_CTAB_protocol.pdf, 246 KB

Permalink Reply by David E. Schindel on December 16, 2009 at 11:02am

Here is the file with the protocols posted by Karen James and the Plant Working Group.

You can find additional information from and about the Plant Working Group on the CBOL webpage
David

Attachments:

• Informationonbarcodeloci.pdf, 114 KB