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Outsourcing DNA extraction and sequencing
Does anyone have suggestions for service labs who could help us?
Also, if we were to do the work in Indonesia, I want to move to a kit that uses column-based extraction rather than phenol-chloroform protocol. DO people have suggestions for kits that are good for a wide range of species? I usually use Qiagen, but they are on the expensive side.
Thanks so much, Gill
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Replies to This Discussion
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Permalink Reply by Isaac Chow on -
Hi Gill,
Here replying from a commercial lab in Hong Kong, we use in house method for DNA extraction and usually success except few known species where usually with PCR inhibitors.
I would suggest you to try resin based method together with proteinase K or physical homogenization for breaking and releasing the cell wall and DNA. It is a cheap and efficient method. If you have got 3500+ specimens, you really have to consider buying a automatic homogenizer (bullet blender) which could homogenize 24 samples in 2 mins.
If you would like to collaborate and interested to discuss further, please message me.
Isaac
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Permalink Reply by Dr A P Singh on -
You can contact Dr George Thomas, Scientist, Rajiv Gandhi Center of Biotechnology, Trivandrum, Kerala for accomplishing the task on payment basis. You can go to the website, download per forma for sending samples and payment
Dr A P Singh
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Permalink Reply by Gillian Dean on -
Hi Isaac - sorry, for some reason I didn't get a notification that you replied to this thread. Thanks too Dr Singh for your suggestion. We are working with the Canadian Centre for DNA Barcoding at the moment, but we may need more sequencing services soon. If so, I will contact again for further information. Thanks very much! Best, Gill
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