international online community for dna barcoding professionals

Lab Operations

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Come share protocols and troubleshooting experiences, compare techniques, and discuss collaborative efforts.

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Sharing Lab Protocols 7 Replies

One of the biggest goals of the Community Network is to share information among barcoders, and information about lab protocols is a very high priority. If you have documents that describe the…Continue

Started by David E. Schindel. Last reply by Gerald Griffin Oct 14, 2011.

Outsourcing DNA extraction and sequencing 3 Replies

We are looking into possibilities for generating barcode data from 3500 Indonesian plant species and we are considering sending our samples out for DNA extracting and sequencing.Does anyone have…Continue

Started by Gillian Dean. Last reply by Gillian Dean Jul 18, 2011.

electrophoresis equipment 1 Reply

i got an email from someone asking about the type of wire to use in a gel tank - it is platinum wire that should be used. for some reason i cant find the post that was in the email in lab…Continue

Started by Gillian Dean. Last reply by Patricia Escalante Dec 19, 2010.

sequencing slightly degraded DNA 3 Replies

Hello,If one amplifies CO1 in two parts with interior primers. Would it be possible to sequence the two parts together in one step?I am asking because we assembling a plate with PCR products of 74…Continue

Started by Patricia Escalante. Last reply by Kris Jett Dec 9, 2010.

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Comment by Wonhoon, Lee on December 27, 2010 at 12:31am: Hi. I’m Wonhoon and working as postdoctoral position at Seoul National University (South Korea). I’m interesting in Korean Forest Insect Pests DNA barcoding. Until now, I have made barcode sequences about 200 species belong to six orders. I hope to learn many developed methods and share my experiences related in barcode researches in this community.

Thank you very much.

Wonhoon Lee

Comment by Thomas Horn on August 28, 2010 at 4:01pm: Hi, i am currently confronted with very weak matK amplification of some species. We use dried material to extract DNA from. I am still not convinced that the extraction is the problem.
Did your group solve their problems?

Comment by Diego Pignataro on March 2, 2010 at 4:11pm: Hi!
My group is having some problems with matK amplification for fresh and herbarium samples, anybody have a recommendation or a protocol to follow? to improve the PCR reaction?

thanks a lot

Diego