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Fungi Discussions (16)

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Definition of an acceptable Fungal ITS barcode

Hi all, We need to get some thoughts on details of how we want to deal with ITS barcodes. I post a first draft of conditions we want to con…

Started by Conrad Schoch

5 Feb 15
Reply by Keith Seifert

Annotation of proteins with Sequin

Hi all, I will try to submit specific info on determining the amino acids for GenBank with Sequin here soon. Feel free to discuss this and…

Started by Conrad Schoch

2 Oct 26, 2011
Reply by Conrad Schoch

Collecting macrofungi for barcoding

Hi all   I am about to go on an official barcoding trip in South Africa. Besides the small ascomycetes that is more my specialty, I will al…

Started by Marieka Gryzenhout

1 Oct 3, 2011
Reply by Bevan Weir

GenBank submission of sequences from barcoding paper

Hi,   What was the final verdict for submitting the sequences that we produced for the barcoding paper? Are we supposed to do it ourselves…

Started by Bevan Weir

1 Jul 28, 2011
Reply by Conrad Schoch

Problems with ITS

So far it looks like we have enough data to recommend ITs as barcode for fungi and we discussed this in some detail in Amsterdam. If you ha…

Started by Conrad Schoch

9 Jul 27, 2011
Reply by Arthur Schuessler

Species and barcoding (Thorsten's proposed collaborative project)

I though it is probably more efficient to start a discussion around Thorsten species delimitation suggestion. I  pasted his email again bel…

Started by Conrad Schoch

3 May 23, 2011
Reply by Laszlo G. Nagy

Defining the boundaries of the ITS barcode

Many people truncate their ITS sequences to begin with the last bases of the 18S, which facilitates alignment with some algorithms:   CATTA…

Started by Keith Seifert

5 May 18, 2011
Reply by Bryn T. M. Dentinger

PCR protocol

Hi Conard, As per your suggestion, I am hereby adding a PCR protocol for MCM7 gene. Best primer combinations and annealing temperature: Fo…

Started by Pradeep Kumar Divakar

11 May 11, 2011
Reply by Conrad Schoch

RPB1 and 2 problems

It seems that several people are having problesm amplifying RPB1 and RPB2. please use this to discuss your issues here. This is stating the…

Started by Conrad Schoch

15 May 6, 2011
Reply by Arthur Schuessler

Mortierella exposed

  Speaking of ITS sequencing of types, here's a real dynamite study: Nagy et al New Phytol, in press (http://onlinelibrary.wiley.com/doi/10

Started by Henrik Nilsson

3 May 1, 2011
Reply by Conrad Schoch

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