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Field Operations

Creating barcode reference records often begins with a collecting event in the field, but without the right techniques the specimen may never qualify as a high-quality barcode record. Come exchange information on field protocols!

Members: 30
Latest Activity: Aug 25, 2013

Discussion Forum

Preservation of jellies 3 Replies

Do the Preservation of gelatinous zooplankton (jellies and siphonophores) in ethanol require any special method.

Started by jagadeesan. Last reply by jagadeesan Feb 3, 2011.

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Comment by Arthur Schuessler on October 23, 2010 at 6:10pm
Dear all, we are also sampling (plant roots with colonizing fungi) and directly storing them in 80% EtOH. We later store at -20°C, also in 80% EtOH. Is there a comparison between storage in EtOH and DMSO? Differences in DNA extraction efficiency?
I have not found the details (yet) and I have to admitt that I never thought about DMSO storage, before. We could transfer all our samples in DMSO, if this is proven to be the better alternative.
Thanks for any information, Arthur
Comment by MOHAMMAD-MONZOOR on July 7, 2010 at 2:45am
Birgit: my e-mail and telephone addresses are adewoleakinwale@yahoo.co.uk and woleakinwale@gmail.com; +234 803 707 9802
Comment by Birgit Gemeinholzer on July 6, 2010 at 5:12am
Sorry - where do I find your email addresses to send you the manual to - mine is b.gemeinholzer@bgbm.org
Comment by MOHAMMAD-MONZOOR on July 5, 2010 at 10:54pm
Hi,
Birgit, kindly forward to me a copy of the field manual. Thanks.
Comment by Vincent Obanda on July 5, 2010 at 8:26am
Hi Birgit, please forward me the copy. thank you
Comment by Birgit Gemeinholzer on July 5, 2010 at 8:00am
Everyone who is interested in the field manual and cannot download ist may contact me - I can forward it.
Comment by MOHAMMAD-MONZOOR on June 30, 2010 at 5:00pm
Thanks, we shall await the final release of the entire document.
Comment by David E. Schindel on June 30, 2010 at 2:35pm
The EDIT ATB+I (All Taxa Biodiversity Inventory and Monitoring) Manual is in the final stages of editing and it should be published at the end of this summer. It will be published by Abc Taxa in Belgium as an open access document about 660 pages long with contributions from about 80 authors. You can see some of the chapters online now at http://www.abctaxa.be/volumes/ but the site requires that you create an online access account before you can look at the manual (Volume 8).
Comment by MOHAMMAD-MONZOOR on June 29, 2010 at 7:52am
Dear David Schindel: could I have a copy of the EDIT-generated methods manual promised to for release since March, 2010. It may be of great assistance to those of us working in Nigeria. Thanks
Comment by Vincent Obanda on June 24, 2010 at 3:39am
Hi Weigt,

Thanks for the insight on the DMSO. I think as you suggest DMSO is superior preservative for DNA compared to 70% ethanol and especially if intention is to ship tissue samples.
 

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