Saturation of a run

Hello

NIce to see some of you at Adelaide, and the research is awesome.  We pyrosequence fungal endophytes (fungi occurring inside plant tissues) directly from the plant material, and analyse the resulting mini-barcodes.   Is there a method to see that in your run, you have completely saturated a lane, so there may be missing data in the output, or that all of your products are there? This is difficult because we are doing this for the first time, so there is no benchmark to compare to.

Thanks

Marieka