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sequencing issue
Hi everyone,
As suggested by Dr. Mike Trizna I am posting my query as discussion point, so that everybody can see each others comments.
I am working on DNA barcoding of plants. I am using rpoC region and while editing sequences I have encountered with a situation where forward and reverse sequence shows 1 or 2 bases variation at same position for eg all samples with the forward primer shows AA and GCG while with reverse all samples shows AAA and GGC, respectively. This creates a problem while combining the forward and reverse sequence. Is it usual to get such kind of sequence variation with forward and reverse primer of same locus and same sample?
If yes then which one is to be correct sequence and can use for further analysis?
Thanking you
Cheers
Rasika
As suggested by Dr. Mike Trizna I am posting my query as discussion point, so that everybody can see each others comments.
I am working on DNA barcoding of plants. I am using rpoC region and while editing sequences I have encountered with a situation where forward and reverse sequence shows 1 or 2 bases variation at same position for eg all samples with the forward primer shows AA and GCG while with reverse all samples shows AAA and GGC, respectively. This creates a problem while combining the forward and reverse sequence. Is it usual to get such kind of sequence variation with forward and reverse primer of same locus and same sample?
If yes then which one is to be correct sequence and can use for further analysis?
Thanking you
Cheers
Rasika
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