international online community for dna barcoding professionals

Barcoding All Lepidoptera

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Learn more and find out how to join efforts toward assembling a comprehensive DNA barcode library for all species of butterflies and moths

Members: 46
Latest Activity: on Monday

You want to join the iBOL Lepidoptera campaign or to know more about its progress?

Visit the new iBOL website for the Lepidoptera campaign!
www.lepbarcoding.org

This site is the perfect place to track progress and learn more about Lepidoptera DNA barcoding and its workflow. There you will find contact info of campaign coordinators and some hints about how you can possibly get involved in existing campaigns or contribute in developing new ones.

The site gives links to relevant sites and a regularly updated list of DNA barcoding publications, in particular the ones related to moths and butterflies.

Happy 2010 to all!
Rodolphe

Discussion Forum

DNA-friendly killing agents 5 Replies

What are the best DNA-friendly killing agents for Lepidoptera? Freezing and cyanide are the available options I have read about, but what about ammonium carbonate?

Started by Matthew Bowser. Last reply by Matthew Bowser Apr 13, 2011.

Hi 3 Replies

Hi I am about to start my work on Barcoding the Moths of North-East India and could absolutely use any help that I can get.Theworks of Cameron and Whiting, amongst others, have led me to…Continue

Started by Mansi Mungee. Last reply by Mansi Mungee Dec 1, 2010.

ND2 gene

I have problem in finding related information related to ND 2 gene aspart of my literature review. can anybody providing any way to searchthis information. as a result of my finding there are no…Continue

Started by Lim Kean Chong Oct 17, 2010.

Direct sequencing Vs sequencing cloned PCR fragments

I have been in sequencing of DNA barcode region in insect species. Direct sequencing of amplified DNA often results in misreading of first 15-25 nucleotides on the 5' end, resulting in shortened…Continue

Started by Virash Kamal Gupta Jun 19, 2010.

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My Comment Wall

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Comment by Peter Huemer on November 30, 2010 at 11:23am: Highly time to joint this phantastic and challenging group. We will try to add what we can, mainly from alpine areas of Europe which still offer a surprising number of taxonomic problems. With the help of barcodes as an important contribution to integrative taxonomy we will succeed

Comment by Ahmed Rahaman Ademolu on October 23, 2010 at 8:09am: Its great joining this group. As Lepidopterans are also good pollinators, the group will assist in using the barcoding technoology for establishing the diversity of the pollinating insects.

Comment by Axel Hausmann on January 12, 2010 at 4:28am: Right now I have analysed the shape of data for the geometrids of our BFB (Barcoding Fauna Bavarica) project and there are 24 species out of 400 (7%) with deep COI splits, now requiring detailed analysis (in progress) to see whether the splits are referring to cryptic diversity or not. Amazing, as Germany is said to be one of the entomologically best explored countries.

Comment by Gaikwad Swapnil Sopan on January 11, 2010 at 11:44pm: I completely agree with you sir. There is much much work for taxonomist. DNA barcoding will indeed surely boost the subject.

Comment by Rodolphe Rougerie on January 11, 2010 at 11:32pm: Right! 37.5K "species" refers to different names, including interim taxonomy.
I guess that overall, considering cryptic diversity, we must have more than 40K different species on BOLD... but then, many are awaiting description or may match synonyms in need of re-validation.

This means much work for taxonomists and a very strong need for barcodes of type specimens, or "topotypes" at least.

Comment by Axel Hausmann on January 11, 2010 at 3:07pm: Wonderful, Rodolphe!

Is there a price for the contributor of the 1/2 millionth barcode?

The 37500 species refer to different names as entered in the submissions, including interim names (codes), don't they? I wonder to how many BINs this pool corresponds. I would suggest that the number will increase.

Comment by Rodolphe Rougerie on January 11, 2010 at 1:37pm: 400K DNA barcodes of Lepidoptera on BOLD!

Just a short note to point at this nice New Year landmark we have reached with the Lepidoptera campaign.
As of January 2010, almost a quarter of the 165K described species have been barcoded!

Let's keep going and get the world fauna done!

Comment by Gaikwad Swapnil Sopan on December 15, 2009 at 4:18am: Dear All,
Its very good for all Lepidoptera lover. I hope we will continue to discuss many issue via this group. keep posting

Comment by Patricia Escalante on November 18, 2009 at 10:44am: Good, thanks. We'll try that.

Comment by Rodolphe Rougerie on November 18, 2009 at 10:15am: Hi Patricia,
It's great to read your colleagues want to start sampling butterflies in your collection.
We do use the standard CCDB protocols for DNA extraction from legs of pinned/dry specimens.

Amplification with universal LepF1/LepR1 primers works very well for specimens up to 10 years old, but success decreases rapidly for older specimens and we need then to use the LepF1/MLepR1 and MLepF1/LepR1 pairs to target shorter fragments.
This usually works great for samples up to 20-30 years old, depending on the preservation and condition of the specimens.