I am about to start my work on Barcoding the Moths of North-East India and could absolutely use any help that I can get.
works of Cameron and Whiting, amongst others, have led me to thinking
about the possibility of using additional mt genes, apart from the
conventional COI. The data on nad2, nad3 and nad4 seems convincing.
would like to know the limitations or pitfalls of using longer gene
sequences for purpose of establishing phylogeny, since I am targeting
the entire COI amplification as opposed to the 5` half of it.
intend on using an additional nuclear gene, the EF1-alpha and am stuck
at choosing the right primer pairs for it. Ideally a sequence length of
650-700bp should suffice but previous works have sequenced as long as
1280bp for identification. If anyone could clear out my doubts/queries, I
would be grateful.