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I am currently doing a study on DNA barcoding for several species of Malaysian herbal plants for my postgraduate study. The barcodes that I am using are rbcL, matK and trnH-psbA.
In order to upload some of my findings to Genebank and BOLD, I need to do the annotations for the sequences (after I have transferred them into Sequin files). I have no problem for annotation process of my rbcL and matK regions since I can label them for instance like this: 'nucleotide 1-655 = partial rbcL gene'
I am however unsure of how to do annotations for my trnH-psbA regions since it's a non-coding gene. Can I just label them as for instance: 'nucleotdie 1-344 = partial trnH-psbA gene' ...?
Or is there any other way or source of reference that I can look up to for annotation of trnH-psbA?
Any advice is deeply appreciated.
I guess it should be like this.
<1..55 (for example)/product="trnH"
36..310/product="trnH-psbA intergenic spacer"
I see. Thank you for your reply, Mr Suang Rungpragayphan.
So does that mean although trnH-psbA is a noncoding gene, we still have to annotate which is trnH and which is psbA?
Let's say that if I am unsure which region is trnH or psbA from my full sequence, should I skip the annotation process when submittng my sequence to Genbank or BOLD?
Is there any source of reference that I can use for annotation of trnH-psbA gene?
Again, thanks a lot for your reply.