international online community for dna barcoding professionals
Pub: Cryptogamie, Algologie, 2010, 31 (4): 423-433
Author(s): Antonio MANGHISI, Marina MORABITO, Clara BERTUCCIO, Line LE GALL, Arnaud COULOUX, Corinne CRUAUD & Giuseppa GENOVESE
A comprehensive assessment of macroalgal biodiversity was conducted along the
coasts of the Straits of Messina (Italy) using DNA barcoding. This approach confirmed theintroduction of Agardhiella subulata in Cape Peloro lagoon (Messina, Italy), an aquatic system in which shellfish importation and stabulation are widely practiced.
Based on a comparison of rbcL sequences, a sample from lake Ganzirri was shown
to be conspecific with A. subulata from North Carolina (USA).
Suspecting that shells of the oyster Crassostrea gigas constitute the main vector for
introduction of exogenous species, individuals from imported stocks were screened for the presence of epizoic algae, which were then sequenced for the DNA barcode region (5’ end of COI gene). A small specimen growing on an oyster shell was assigned with confidence to A. subulata based on its COI sequence, a task that would have been virtually impossible based on morphological characters of the immature specimen. Based on the example here, we conclude that monitoring algal diversity using DNA barcoding would clearly be a rapid and efficient approach to highlight the introduction of alien species.