international online community for dna barcoding professionals
Greetings, everyone! I am Seethapathy and im working in Institute of Ayurveda and Integrative Medicine, Bangalore, India. As a research scholar, currently developing Molecular markers for the authentication of Medicinal plants from its adulterants and substitutes by using ITS primers.. and i have desire to establish this work up to DNA barcoding. im keep on reading the published articles and other datas about Barcoding.
I would be glad to know whether anybody else may be interested in DNA Barcoding for the medicinal plants used in Ayurveda i could be useful for me...
Hello Dr. Seethapathy
I am working of fish taxonomy. the question Is ITS used to fish DNA befor.
It is a good to use it for this purpose.
greeting to all
But My best wishes to Kris
i am Amit Patidar.i am a student.recently i have completed my master's in Bioinformatic.i am curious to join dna bar coding group.it will be a milestone for my carrier.
I express my sincere delight in joining the barcode community.
Hi my name is Alison Dann I have recently started a job in Tasmania's Department of Primary Industries in Plant Health and Biosecurity and first job on my list is to PCR and sequence various plant pathogenic fungal collections that have been stored for years here in Tasmania, specifically, Chrysomyxa spp., Fusarium spp. and a Stromatinia gladioli strain.
I have found the BOLD website extremely helpful in finding papers and primers, as I come from a bacterial/viral background, I did not have any idea on what to use for fungal DNA sequencing. Thanks and hope to contribute in the future.
You are strating with a new field particularly from thepoint that you have been working with bacterial/ viral. wrt fungus, the nuclei are concentrated more towards growing tips with following mycelial filaments with progressively decreasing number of nuclei- mean less nuclei from non actively growing mycelia. For DNA isolation you need to grind fresh mycelial mats in liquid Nitrogen and extract DNA using CTAB method used in plant DNA isolation. For DNA barcoding ITS sequences have been defined by IBOL/ BOLD methodology. So go into available information and finalize your programs. all the best.
I am John Olayinka ATOYEBI
Research Scientist, National Centre for Genetic Resources and Biotechnology, Ibadan, Nigeria.
Particular interest in utilising DNA barcode for species identification in the Centre. The Centre is an active member of the CBOL, but hope to do more from now, into actual laboratory practices and analysis of DNA barocde data.
My name is Haslawati Baharuddin, I am a research officer affiliated to the Fisheries Research Institute (Freshwater Division), Department of Fisheries, Malaysia. I am currently with inland fisheries program, working mostly on taxonomic identification of freshwater fishes, mostly indigenous species. As barcoding is part of taxonomic tool for species identification, therefore I hope this group will help me in solving some of my taxonomic questions especially when dealing with the impacts of exotic species introduction. Hope to get the most from this group...
Another thing, apart from doing COI, I am also trying to incorporate nuclear gene RAG 1 for my data set. However, it is hard to get good PCR band on most of the DNA samples. I managed to get one band for one sample, but unfortunately the sequence was so bad that I hardly could read them. I am using primers from Lopez et al (2004). Can anyone out there share their experience with me please? Thank you
great to know about your work, NE region is difficult for biodiversity studies, in this regard your work will be useful. I am interested in knowing how many catfish species you had, please share this information. Also, we are looking for C. batrachus population from NE. if it is available, please inform me.
I have communicated a paper. You wll find all your queries on the paper very soon....