international online community for dna barcoding professionals

Introductions

Welcome to Connect, the DNA Barcoder community network!

We hope this network will be a place for communication and collaboration on a global scale, overcoming the rather dispersed nature of DNA barcoder researchers and enthusiasts.

As a first step, why not introduce yourself to the community via the comment box below? Including your name, institution, interest in DNA barcoding, and what you hope to get from this community would be a great way to start.

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Replies to This Discussion

Permalink Reply by Seethapathy GS on June 2, 2011 at 6:33am

Hello!

Greetings, everyone! I am Seethapathy and im working in Institute of Ayurveda and Integrative Medicine, Bangalore, India. As a research scholar, currently developing Molecular markers for the authentication of Medicinal plants from its adulterants and substitutes by using ITS primers.. and i have desire to establish this work up to DNA barcoding. im keep on reading the published articles and other datas about Barcoding.

I would be glad to know whether anybody else may be interested in DNA Barcoding for the medicinal plants used in Ayurveda i could be useful for me...

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Permalink Reply by Dr. Mustafa S. Faddagh on June 3, 2011 at 8:16am

Hello Dr. Seethapathy

I am working of fish taxonomy. the question Is ITS used to fish DNA befor.

It is a good to use it for this purpose.

Thank you

greeting to all

But My best wishes to Kris

sam

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Permalink Reply by amit patidar on June 12, 2011 at 11:25pm

hiii.

i am Amit Patidar.i am a student.recently i have completed my master's in Bioinformatic.i am curious to join dna bar coding group.it will be a milestone for my carrier.

I express my sincere delight in joining the barcode community.

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Permalink Reply by Chrissen E. C. Gemmill on June 15, 2011 at 5:24pm

Kia ora everyone, I am Chrissen and have been working in New Zealand at the University of Waikato for 13 years now. Originally from the USA, I did my PhD at University of Colorado and my postdoc at the Smithsonian. I am a botanist/molecular systematist interested in the evolution and biogeography of pacific island plants. My colleagues (Munzinger, Lowry, Plunkett, Buerki, et al.) and I are working from the species to order level in the Apiales (in particular I am interested in Pittosporum) and focusing on the evolution of the New Caledonian flora; plants of Zealandia including the Winteraceae and Lauraceae. With colleagues (Cary, Hicks, Kelly et al.; King, Banks) I am also involved in using molecular tools to unravel invasions of organisms into New Zealand (invasive diatom Didymosphenia geminata, even house mice); I have worked on Antarctic mosses and also conservation genetics of island plants in Hawaiian and New Zealand (Brighamia, Pritchardia, Sesbania, etc; Zostera, Pittosporum, etc). I am also interested in restoration/ecosourcing genetics (Clark, Stevens, et al.). I would especially love to hear from others working on island plants and the markers they are finding useful in addition to ITS to identify species, assess species delimitations and reconstruct relationship among closely related plants.

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Permalink Reply by Alison Dann on June 21, 2011 at 12:43am

Hi my name is Alison Dann I have recently started a job in Tasmania's Department of Primary Industries in Plant Health and Biosecurity and first job on my list is to PCR and sequence various plant pathogenic fungal collections that have been stored for years here in Tasmania, specifically, Chrysomyxa spp., Fusarium spp. and a Stromatinia gladioli strain.

I have found the BOLD website extremely helpful in finding papers and primers, as I come from a bacterial/viral background, I did not have any idea on what to use for fungal DNA sequencing. Thanks and hope to contribute in the future.

cheers Alison

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Permalink Reply by Virash Kamal Gupta on June 21, 2011 at 3:28am

Dear Alison,

You are strating with a new field particularly from thepoint that you have been working with bacterial/ viral. wrt fungus, the nuclei are concentrated more towards growing tips with following mycelial filaments with progressively decreasing number of nuclei- mean less nuclei from non actively growing mycelia. For DNA isolation you need to grind fresh mycelial mats in liquid Nitrogen and extract DNA using CTAB method used in plant DNA isolation. For DNA barcoding ITS sequences have been defined by IBOL/ BOLD methodology. So go into available information and finalize your programs. all the best.

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Permalink Reply by Alison Dann on June 21, 2011 at 7:06pm

Thanks so much for the help and information, I will do that!

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Permalink Reply by John Olayinka ATOYEBI on July 6, 2011 at 10:37am

Hello,

I am John Olayinka ATOYEBI

Research Scientist, National Centre for Genetic Resources and Biotechnology, Ibadan, Nigeria.

Particular interest in utilising DNA barcode for species identification in the Centre. The Centre is an active member of the CBOL, but hope to do more from now, into actual laboratory practices and analysis of DNA barocde data.

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Permalink Reply by Haslawati Baharuddin on July 14, 2011 at 12:38am

Hello everybody,

My name is Haslawati Baharuddin, I am a research officer affiliated to the Fisheries Research Institute (Freshwater Division), Department of Fisheries, Malaysia. I am currently with inland fisheries program, working mostly on taxonomic identification of freshwater fishes, mostly indigenous species. As barcoding is part of taxonomic tool for species identification, therefore I hope this group will help me in solving some of my taxonomic questions especially when dealing with the impacts of exotic species introduction. Hope to get the most from this group...

Another thing, apart from doing COI, I am also trying to incorporate nuclear gene RAG 1 for my data set. However, it is hard to get good PCR band on most of the DNA samples. I managed to get one band for one sample, but unfortunately the sequence was so bad that I hardly could read them. I am using primers from Lopez et al (2004). Can anyone out there share their experience with me please? Thank you

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Permalink Reply by Maloyjo Joyraj Bhattacharjee on July 16, 2011 at 12:15pm

Hello everyone, I am Maloyjo Joyraj Bhattacharjee and I work on DNA barcoding of catfishes of Northeast India. However, simply undergoing barcoding I also work on population genomics. My current interest is to overhaul the technique further critically analysing the barcode of a large set of database.

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Permalink Reply by Gulab D Khedkar on July 18, 2011 at 1:33am

Dear Maloyjo,

great to know about your work, NE region is difficult for biodiversity studies, in this regard your work will be useful. I am interested in knowing how many catfish species you had, please share this information. Also, we are looking for C. batrachus population from NE. if it is available, please inform me.

Best wishes,