international online community for dna barcoding professionals
Introductions
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Replies to This Discussion
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Permalink Reply by David E. Schindel on -
Greetings, everyone! I'm David Schindel, Executive Secretary of CBOL, the Consortium for the Barcode of Life. I hope by now everyone understands the relationship between CBOL and iBOL, the International Barcode of Life project. CBOL promotes barcoding through working groups, workshops, training, outreach, the international conference held every two years, and lots of other activities. What we don't do is barcoding - we aren't directly involved in specimens, labs, data, etc. but we help other people do this. CBOL sponsors the Connect community network for this purpose. iBOL, on the other hand, is the largest barcoding project with a goal of 5 million barcode records from 500,000 species.
Here are some of the things on my mind:
- How can we use the Connect network to get people together for barcoding projects?
- How can we help the iBOL Working Groups get off to a fast start?
- How can we get people to use Connect more actively? What rewards/incentives should we offer?
- What standard barcode regions should be approved for fungi, protists, and other taxonomic groups for which COI doesn't work? (We've already launched working groups for fungi and protists.)
- We're going to start holding webinars through the Connect network soon and you'll be getting notices. What webinar topics would you like to see?
Let's hear from you out there!!
David Schindel
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Permalink Reply by Muhammad Atique Akhter on -
Hi Everyone,
I am from Pakistan, and worling on Taxonomy of Elateridae (Coleoptera) from Pakistan, i am intrested to do barcoding of Pakistani elaterids, could anyone help me.
is it possible online training ? or i send specimens to someone?
Thanks to allow me to join.
Muhammad Atique Akhter
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Permalink Reply by SANGITA SHRESTHA on
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Dear Dr. Schindel,
I'm now in the network. Please let me know how I can equip myself with DNA barcoding techniques as I have to supervise a number of B. Sc., M. Sc. and Ph. D. Biotech students working under me and my colleagues in barcoding projects of Nepalese medicinal plants and prospective microbes (fungi and bacteria). Thanking you.
with best regards
Sangita Shrestha, PhD
NAST
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Permalink Reply by Virash Kamal Gupta on -
Dear Dr Sangita,
We are into barcoding of agriculturally important insect species in Punjab. Default genes for DNA barcoding of plants are 'matK' and 'rbcL', for bacteria it is '16s ribosomal DNA', for fungi it is ITS spacer region situated between the 18S (SSU) and 28S (LSU) genes in the nrDNA repeat. You can find sequences from internet/ BOLD site.
Most appropriate and economical for you will be following steps.
1. DNA isolation from different organisms.
2. Get the primers synthesized by custom facilities.
3. Standardize PCR amlification conditions and purification of PCR product of desired size.
Most scientists go for Direct sequencing of PCR product (unpurified). However I have found following problem
i. The secondary bands in PCR product interfere with sequencing to give either no results or poor results.
ii. even in the best case of sequencing results on the first 20-25 bases are lost.
I prefer PCR cloning of PCR products in a PCR cloning vector in which cloning site is bordered by M13 sequencing primers. This takes care of sequence data that is lost during sequencing on first bases. The sequence is always the best.
3. Submit bacterial clones in stabs direct to the custom sequencing facilities (many are available in India, But best is Xcelris, Ahmedabad). This will save you from reisolating and purifying recombinant plasmids for sequencing.
I am sure you are a molecular biologist and will be able to understand and adapt to my suggestions. All the best.
Dr V K Gupta, PhD
Department of Entomology
PAU, Ludhiana
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Permalink Reply by Kris Jett on -
Hello!
My name is Kris Jett, and I'm in charge of web and print outreach for the Consortium for the Barcode of Life, based in the Smithsonian Institution's National Museum of Natural History in Washington D.C., USA.
I don't get to do any science myself, but I love learning about all the different applications that innovative researchers have found for DNA barcoding.
I'm hoping that this community will help me learn more about researchers' needs, and will be a key point for communication and coordination of events, workshops, training, and anything else that will help strengthen and promote DNA barcoding. If you ever have any questions about how to share resources, make connections, or use the network, just let me know and I'll be happy to help as best I can!
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Permalink Reply by Tushar on
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Dear Kris
Just heard that one can avail a short term training at CBOL! Is it true?
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Permalink Reply by Kris Jett on
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Dear Tushar,
Unfortunately, CBOL can't directly provide support and training. However, CBOL's Leading Labs Network (http://connect.barcodeoflife.net/group/LLN) sometimes have fellowship and training opportunities available. I would recommend you join the group and post a comment specifying what training opportunities you're looking for. Even if the LLN doesn't have anything planned, chances are they will know of some resource that will help.
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Permalink Reply by Rodolfo Barreiro on -
Hi,
This is Rodolfo Barreiro. I work at the Universidade da Coruña (Spain) in collaboration with a team of marine (seaweeds) botanists (I. Bárbara, J. Cremades, V. Peña. P. Díaz). We are currently considering the use of barcodes in our work. We would be glad to know whether anybody else may be interested in the same taxa and geographical region (European Atlantic).
Regards,
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Permalink Reply by Dina N Kovarik on -
Hello! My name is Dina Kovarik, Program Manager for the Bio-ITEST Program at the Northwest Association for Biomedical Research (NWABR). We are partnering with Digital World Biology, the EdLab Group, Seattle Aquarium and high school teachers from around the country to develop and disseminate high school science curricula about bioinformatics, barcoding, and evolution. Our work is funded by the Innovative Technology Experiences for Students and Teachers (ITEST) program through the National Science Foundation, and includes curriculum development, professional development workshops for teachers, and on-going support for teachers and students in their classrooms. The Seattle Aquarium has graciously agreed to provide samples for authentic student research barcode projects. We are very excited to be part of this Barcode of Life community, to learn more about barcoding projects, and connect with others who would like to be involved with our work.
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Permalink Reply by Lyndell M. Bade on -
Hi Dina,
That sounds like a great project...how is it going? I'd love to hear more! I did avian ecology and reproductive ecology research with high school students in MO, and that was so rewarding! What species have you all worked with to date? I'm about to start sequencing my shellfish species here in NC. Are your high school students actually doing the sequencing? Or are they working with other researchers at local universities? I'm just interested in connecting with people who are/have been barcoding marine species, especially bivalves. If you know of anyone with experience with those species, I'd love a referral/introduction.
Best,
Lyndell
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Permalink Reply by Sandra Lorena Ament Velásquez on -
Hi! I'm Sandra Lorena Ament, I'm starting to work at the Genetics and Evolution laboratory under the guidence of Daniel Piñero, in the Instituto de Ecología of the Universidad Nacional Autónoma de México. My proyect involves lichens of the genus Usnea and I'll try to use barcoding. I'm just starting so I hope I can get some help :D!
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Permalink Reply by meenakshi sharma on
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hello I am meenakshi planning to work on spiders I want to do barcodind of the spider which i am having but i am not getting the way out. I am a PH D student in India.
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