international online community for dna barcoding professionals
Introductions
Welcome to Connect, the DNA Barcoder community network!
We hope this network will be a place for communication and collaboration on a global scale, overcoming the rather dispersed nature of DNA barcoder researchers and enthusiasts.
As a first step, why not introduce yourself to the community via the comment box below? Including your name, institution, interest in DNA barcoding, and what you hope to get from this community would be a great way to start.
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Replies to This Discussion
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Permalink Reply by David E. Schindel on -
Greetings, everyone! I'm David Schindel, Executive Secretary of CBOL, the Consortium for the Barcode of Life. I hope by now everyone understands the relationship between CBOL and iBOL, the International Barcode of Life project. CBOL promotes barcoding through working groups, workshops, training, outreach, the international conference held every two years, and lots of other activities. What we don't do is barcoding - we aren't directly involved in specimens, labs, data, etc. but we help other people do this. CBOL sponsors the Connect community network for this purpose. iBOL, on the other hand, is the largest barcoding project with a goal of 5 million barcode records from 500,000 species.
Here are some of the things on my mind:
- How can we use the Connect network to get people together for barcoding projects?
- How can we help the iBOL Working Groups get off to a fast start?
- How can we get people to use Connect more actively? What rewards/incentives should we offer?
- What standard barcode regions should be approved for fungi, protists, and other taxonomic groups for which COI doesn't work? (We've already launched working groups for fungi and protists.)
- We're going to start holding webinars through the Connect network soon and you'll be getting notices. What webinar topics would you like to see?
Let's hear from you out there!!
David Schindel
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Permalink Reply by Muhammad Atique Akhter on -
Hi Everyone,
I am from Pakistan, and worling on Taxonomy of Elateridae (Coleoptera) from Pakistan, i am intrested to do barcoding of Pakistani elaterids, could anyone help me.
is it possible online training ? or i send specimens to someone?
Thanks to allow me to join.
Muhammad Atique Akhter
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Permalink Reply by Kris Jett on -
Hello!
My name is Kris Jett, and I'm in charge of web and print outreach for the Consortium for the Barcode of Life, based in the Smithsonian Institution's National Museum of Natural History in Washington D.C., USA.
I don't get to do any science myself, but I love learning about all the different applications that innovative researchers have found for DNA barcoding.
I'm hoping that this community will help me learn more about researchers' needs, and will be a key point for communication and coordination of events, workshops, training, and anything else that will help strengthen and promote DNA barcoding. If you ever have any questions about how to share resources, make connections, or use the network, just let me know and I'll be happy to help as best I can!
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Permalink Reply by Tushar on
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Dear Kris
Just heard that one can avail a short term training at CBOL! Is it true?
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Permalink Reply by Kris Jett on
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Dear Tushar,
Unfortunately, CBOL can't directly provide support and training. However, CBOL's Leading Labs Network (http://connect.barcodeoflife.net/group/LLN) sometimes have fellowship and training opportunities available. I would recommend you join the group and post a comment specifying what training opportunities you're looking for. Even if the LLN doesn't have anything planned, chances are they will know of some resource that will help.
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Permalink Reply by Rodolfo Barreiro on -
Hi,
This is Rodolfo Barreiro. I work at the Universidade da Coruña (Spain) in collaboration with a team of marine (seaweeds) botanists (I. Bárbara, J. Cremades, V. Peña. P. Díaz). We are currently considering the use of barcodes in our work. We would be glad to know whether anybody else may be interested in the same taxa and geographical region (European Atlantic).
Regards,
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Permalink Reply by Dina N Kovarik on -
Hello! My name is Dina Kovarik, Program Manager for the Bio-ITEST Program at the Northwest Association for Biomedical Research (NWABR). We are partnering with Digital World Biology, the EdLab Group, Seattle Aquarium and high school teachers from around the country to develop and disseminate high school science curricula about bioinformatics, barcoding, and evolution. Our work is funded by the Innovative Technology Experiences for Students and Teachers (ITEST) program through the National Science Foundation, and includes curriculum development, professional development workshops for teachers, and on-going support for teachers and students in their classrooms. The Seattle Aquarium has graciously agreed to provide samples for authentic student research barcode projects. We are very excited to be part of this Barcode of Life community, to learn more about barcoding projects, and connect with others who would like to be involved with our work.
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Permalink Reply by Sandra Lorena Ament Velásquez on -
Hi! I'm Sandra Lorena Ament, I'm starting to work at the Genetics and Evolution laboratory under the guidence of Daniel Piñero, in the Instituto de Ecología of the Universidad Nacional Autónoma de México. My proyect involves lichens of the genus Usnea and I'll try to use barcoding. I'm just starting so I hope I can get some help :D!
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Permalink Reply by meenakshi sharma on
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hello I am meenakshi planning to work on spiders I want to do barcodind of the spider which i am having but i am not getting the way out. I am a PH D student in India.
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Permalink Reply by Julie Stahlhut on -
Dear Meenakshi,
This URL links to an article on spider barcoding. A brief protocol is given on page 32 of the article (which is page 6 of the PDF.)
http://pensoftonline.net/zookeys/index.php/journal/article/view/239...
The description is very brief, but the authors used two different primer sets that are very commonly used here at BIO. One was the "tailed Folmer" set, LCO1490_t1 + HCO2198_t1. The products are then sequenced with M13 forward and reverse sequencing primers. The other pair was LepF1 + LepR1, which were developed for Lepidoptera but work well in many other arthropods. These products are sequenced with the same primers used for amplification.
You can find information about these primers at the URL below:
http://www.boldsystems.org/views/primerlist.php
Best wishes for a successful project!
-- Julie Stahlhut
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Permalink Reply by meenakshi sharma on
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Dear Julie
thanks for the reply . The link which you had send me thats very helpful but the barcoding requires a 96 well plate setting do you have a idea from where i could get that set up
Meenakshi
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Permalink Reply by Julie Stahlhut on
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Dear Meenakshi,
I'm not sure what you mean by a 96-well plate setting. Are you going to send specimens to a different laboratory, or are you doing the sequencing reactions yourself?
-- Julie
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