Ideal collection fluid for Thrips tabacci

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Permalink Reply by Lee Weigt on May 9, 2011 at 3:30pm

there is no ideal collection fluid - every one has some drawbacks.

it would help to know more specifics on what you tried and what the results were.

types of ethanol, what the spectrophotometer measurements and gel images of the extracted DNA were, etc

 

in general:

water - NO

ethanol - yes, if both non-denatured and 95% or greater; but shipping problems potentially.

RNA later ,yes, in theory.

 

salt buffer - yes, recipe below

 

for all: helps to make sure you are preserving the right body parts, and in timely fashion.

 

Salt/DMSO Storage Buffer Recipe

20% DMSO

0.25M sodium-EDTA

saturated NaCl

pH 7.5

Citation: Seutin, G., B.N. White and P.T. Boag.  1991. Preservation of avian blood and tissue samples for DNA analyses.  Can. J. Zool. 69: 82-90.

 

 

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Permalink Reply by Amol Bharat Ghodke on May 9, 2011 at 10:32pm

Thanks lee.... it's going to help me all the way....

Regards

Amol

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