I am currently working on zooplankton diversity in freshwater bodies, and there is some setback w.th respect to successful amplification of mitochondrial genome for Copepoda and cyclopoida (from 5 days- 1year old sample).
Can anyone provide there insight for finding solution to the problem.
Insight to genetic setup of my study:
DNA extraction: Qiagen DNA kit
Taq polymerase: Platinum Taq
Sample preserved in : 70% denatured Ethanol.