Does DNA isolated using commercial kits degrade fast..

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Permalink Reply by Isaac Chow on January 18, 2011 at 9:14am

DNA/ RNA don't degrade that rapid itself, except the presence of certain DNase/ RNase and that could be produced by bacteria with un-intentional contamination.

 

DNA extraction often involve the use of ethanol and Guanidine HCl, which would rule out the proteins and unwanted bacterium, however, if you re-suspend them in inappropriate buffer, eg. plain H2O, and storing at ambient temperature, more likely you will have bacterial growth and thats why AVE in Qiagxn (Viral RNA extraction kit) is added with sodium azide and thats to prevent unwanted growth of microorganism.

 

Hope this can help.

 

tips: I have experience with recovery of DNA with commercial kits storing at 4 degree Celsius and able to be amplified after several years 

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